PMID: 8961292Nov 15, 1996Paper

Chromaffin granule membrane glycoprotein IV is identical with Ac45, a membrane-integral subunit of the granule's H(+)-ATPase

Neuroscience Letters
F GetlawiD K Apps

Abstract

Glycoprotein IV of bovine adrenal chromaffin granule membranes was purified by membrane fractionation with Triton X-114 and lectin affinity chromatography. An antiserum raised against this protein recognized the same component as one directed against subunit Ac45 of the proton-translocating adenosine triphosphatase in the granule membrane. Amino acid sequencing confirmed that glycoprotein IV and Ac45 are identical proteins, and also showed that they are derived from a larger precursor by removal of a 246-amino acid N-terminal sequence. Enzymatic deglycosylation indicated an apparent polypeptide molecular mass of 29 kDa for the mature Ac45/glycoprotein IV. Blue Native electrophoresis confirmed that this protein is a component of the membrane sector of the V-ATPase.

References

Jan 1, 1979·Journal of Neurochemistry·E HuberH Winkler
Oct 1, 1989·The Biochemical Journal·D K AppsJ R Perez-Castineira
Aug 15, 1996·European Journal of Biochemistry·L C Webster, D K Apps

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Citations

May 21, 1999·European Journal of Biochemistry·J C HolthuisG J Martens
Aug 21, 2008·Proceedings of the National Academy of Sciences of the United States of America·Els LouagieJohn W M Creemers
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·F Stephen HodiGlenn Dranoff
Aug 13, 2010·Molecular Biology of the Cell·Eric J R JansenGerard J M Martens
Dec 1, 2009·Cellular and Molecular Life Sciences : CMLS·Eric J R JansenGerard J M Martens
May 22, 2009·Annals of the New York Academy of Sciences·W J J M ScheenenG J M Martens
Apr 30, 1999·Physiological Reviews·N Nelson, W R Harvey
Apr 16, 2002·European Journal of Biochemistry·Vincent Th G SchoonderwoertGerard J M Martens
Jul 29, 2008·Biochimica Et Biophysica Acta·Eric J R JansenGerard J M Martens

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