Chromatin-associated genes protect the yeast genome from Ty1 insertional mutagenesis.

Genetics
Katherine M NyswanerDavid J Garfinkel

Abstract

Chromosomal genes modulate Ty retrotransposon movement in the genome of Saccharomyces cerevisiae. We have screened a collection of 4739 deletion mutants to identify those that increase Ty1 mobility (Ty1 restriction genes). Among the 91 identified mutants, 80% encode products involved in nuclear processes such as chromatin structure and function, DNA repair and recombination, and transcription. However, bioinformatic analyses encompassing additional Ty1 and Ty3 screens indicate that 264 unique genes involved in a variety of biological processes affect Ty mobility in yeast. Further characterization of 33 of the mutants identified here show that Ty1 RNA levels increase in 5 mutants and the rest affect mobility post-transcriptionally. RNA and cDNA levels remain unchanged in mutants defective in transcription elongation, including ckb2Delta and elf1Delta, suggesting that Ty1 integration may be more efficient in these strains. Insertion-site preference at the CAN1 locus requires Ty1 restriction genes involved in histone H2B ubiquitination by Paf complex subunit genes, as well as BRE1 and RAD6, histone H3 acetylation by RTT109 and ASF1, and transcription elongation by SPT5. Our results indicate that multiple pathways restrict Ty1 mobi...Continue Reading

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Citations

Sep 2, 2009·Proceedings of the National Academy of Sciences of the United States of America·Emiko Matsuda, David J Garfinkel
Apr 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Leslie MitchellKristin Baetz
Jul 14, 2009·Genome Research·Célia PayenCécile Neuvéglise
Aug 6, 2008·Molecular and Cellular Biology·Francisco Malagon, Torben Heick Jensen
Aug 6, 2008·Annual Review of Genetics·Arthur BeauregardMarlene Belfort
Aug 4, 2012·Mobile DNA·Jenni K RislerM Joan Curcio
Nov 7, 2008·PLoS Biology·Sasha F Levy, Mark L Siegal
May 6, 2010·PLoS Genetics·Reinhard BrunmeirChristian Seiser
Oct 27, 2010·PLoS Genetics·Ann DemoginesSara L Sawyer
Oct 30, 2008·Genetics·Chun DongJeffrey S Han
May 26, 2010·Genetics·Arun DakshinamurthyDavid J Garfinkel
Sep 25, 2012·Traffic·Mary Ann CheckleyDavid J Garfinkel
Aug 9, 2011·Comptes rendus biologies·Claudine Bleykasten-Grosshans, Cécile Neuvéglise
Sep 2, 2015·Briefings in Functional Genomics·Vivien Measday, Peter C Stirling
Jan 28, 2015·RNA Biology·Michael P Guy, Eric M Phizicky
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Jan 17, 2020·Nucleic Acids Research·Margarita T AngelovaClément Carré
May 2, 2018·Mobile DNA·Stephanie CheungVivien Measday

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