Chromatin de-condensation by switching substrate elasticity

Scientific Reports
Morgane RabineauDominique Vautier

Abstract

Mechanical properties of the cellular environment are known to influence cell fate. Chromatin de-condensation appears as an early event in cell reprogramming. Whereas the ratio of euchromatin versus heterochromatin can be increased chemically, we report herein for the first time that the ratio can also be increased by purely changing the mechanical properties of the microenvironment by successive 24 h-contact of the cells on a soft substrate alternated with relocation and growth for 7 days on a hard substrate. An initial contact with soft substrate caused massive SW480 cancer cell death by necrosis, whereas approximately 7% of the cells did survived exhibiting a high level of condensed chromatin (21% heterochromatin). However, four consecutive hard/soft cycles elicited a strong chromatin de-condensation (6% heterochromatin) correlating with an increase of cellular survival (approximately 90%). Furthermore, cell survival appeared to be reversible, indicative of an adaptive process rather than an irreversible gene mutation(s). This adaptation process is associated with modifications in gene expression patterns. A completely new approach for chromatin de-condensation, based only on mechanical properties of the microenvironment, wi...Continue Reading

References

Jun 6, 2002·Trends in Genetics : TIG·Niall Dillon, Richard Festenstein
Jan 14, 2003·Biomacromolecules·Jonas D MendelsohnMichael F Rubner
Mar 8, 2003·The Journal of Biological Chemistry·Karanam BalasubramanyamTapas K Kundu
Sep 3, 2004·Nature Reviews. Cancer·Daniele ZinkJeffrey A Nickerson
Feb 22, 2005·Biomaterials·Cédric BouraPatrick Menu
Sep 20, 2005·Cancer Cell·Matthew J PaszekValerie M Weaver
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Jan 15, 2009·Nature Reviews. Cancer·Alain EychèneCelio Pouponnot
Jan 21, 2009·Cancer Metastasis Reviews·Sanjay Kumar, Valerie M Weaver
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Ning WangDonald E Ingber
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Mar 18, 2009·Bioinformatics·Cole TrapnellSteven L Salzberg
Nov 26, 2009·Cell·Kandice R LeventalValerie M Weaver
Oct 29, 2010·Genome Biology·Simon Anders, Wolfgang Huber
Dec 24, 2010·Nature Reviews. Molecular Cell Biology·Alexandre Gaspar-MaiaMiguel Ramalho-Santos
Jan 7, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Amit Pathak, Sanjay Kumar
Mar 10, 2011·Nature Medicine·Hans Clevers
Oct 20, 2011·Nature Reviews. Gastroenterology & Hepatology·Victoria Valinluck Lao, William M Grady
Dec 23, 2011·Development·Emma J StringerFelix Beck
Jul 4, 2012·Nature Materials·Jing LiuBo Huang
Mar 26, 2013·Nature Reviews. Molecular Cell Biology·Aimee I Badeaux, Yang Shi
Mar 19, 2014·Nature Materials·Chun YangKristi S Anseth
Jun 3, 2014·Molecular Cancer·Xin TangMark S Kuhlenschmidt
Jun 7, 2014·Cell Stem Cell·Anne Laugesen, Kristian Helin
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Mar 5, 2016·Current Opinion in Biotechnology·Fabian SpillMuhammad H Zaman
Feb 12, 2017·Cell·Arthur W LambertRobert A Weinberg
Feb 14, 2018·The Journal of Experimental Medicine·Camille BalbinotIsabelle Duluc

❮ Previous
Next ❯

Citations

Aug 21, 2020·Proceedings of the National Academy of Sciences of the United States of America·Anouk R KillaarsKristi S Anseth
Jun 23, 2021·Biomaterials·Claire EhlingerLeyla Kocgozlu
Apr 29, 2021·Annual Review of Biomedical Engineering·Amr A AbdeenKrishanu Saha
Aug 8, 2021·International Journal of Molecular Sciences·Seung Hee ChoiSuk Weon Kim
Oct 11, 2021·Biophysical Journal·Christopher C PriceVivek B Shenoy

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission electron microscopy
acetylation
electron microscopy
fluorescence microscopy
chips

Software Mentioned

Sigma Plot
ImageJ
Bowtie
Elements
Tophat
Elements Br
HTSeq
DAVID
NIS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nature Methods
Peter Karagiannis, Shinya Yamanaka
Journal of Gerontology
I Wittels, J Botwinick
International Review of Cytology
A Babu, R S Verma
© 2022 Meta ULC. All rights reserved