PMID: 6402757Jan 25, 1983Paper

Chromatin fine structure of the histone gene complex of Drosophila melanogaster

Nucleic Acids Research
A WorcelP Schedl

Abstract

We have used salt extractions of nuclei and long agarose gels to dissect the chromatin fine structure of the histone gene repeat of Drosophila melanogaster. Extraction of nuclei with 0.35 M KCl removes many non-histone chromosomal proteins but does not significantly disturb the overall nucleosome arrangement of the repeat unit. After extraction of nuclei with 0.55 M KCl, which also removes histone Hl, the basic arrangement of nucleosome core particles in the repeat unit is not greatly disturbed and the exposed DNA segments near the 5' ends of the histone genes are also retained. Extraction of nuclei with 0.75 M or higher KCl concentrations causes extensive nucleosome sliding and rearrangement with accompanying changes in the nucleoprotein organization of the histone gene complex and loss of the 5' hypersensitive sites. Our results indicate that the histone gene repeat displays a highly organized chromatin structure in vivo.

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Citations

Dec 1, 1986·Molecular & General Genetics : MGG·J E Pérez-OrtinL Franco
Aug 10, 1984·Nucleic Acids Research·A T Annunziato, R L Seale
Oct 25, 1994·Nucleic Acids Research·L PoljakU K Laemmli
Jan 6, 2011·PloS One·Ho-Ryun ChungMartin Vingron
Dec 5, 1989·Journal of Molecular Biology·E IzaurraldeU K Laemmli
Sep 20, 1986·Journal of Molecular Biology·A UdvardyT S Hsieh
May 15, 1984·Journal of Molecular Biology·A UdvardyP Schedl
Nov 15, 1984·Journal of Molecular Biology·R DeLotto, P Schedl
May 1, 1984·Cell·M Ryoji, A Worcel
Feb 5, 1984·Journal of Molecular Biology·A Udvardy, P Schedl
Nov 5, 1983·Journal of Molecular Biology·G Gargiulo, A Worcel
Jun 5, 1986·Journal of Molecular Biology·I Ruberti, A Worcel
Jun 15, 1984·Journal of Molecular Biology·H BöckT Igo-Kemenes
Aug 20, 1985·Journal of Molecular Biology·S HanP Schedl
Nov 25, 1984·Journal of Molecular Biology·C A Edwards, R A Firtel
Dec 15, 1987·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J MirkovitchU K Laemmli
Apr 1, 1992·Current Opinion in Genetics & Development·U K LaemmliY Adachi

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