Chromatin immunoprecipitation identifies genes under direct VraSR regulation in Staphylococcus aureus

Canadian Journal of Microbiology
Mrittika SenguptaRadheshyam K Jayaswal

Abstract

Transcriptional profiling of Staphylococcus aureus treated with cell wall-active antibiotics identified the 2-component system, VraSR, as one of the key players in response to antibiotic stress. Although it has been shown that a number of genes are regulated by the VraSR system, it has not been shown which genes are under direct VraSR regulation and which genes are not. In this study, chromatin immunoprecipitation techniques were used to identify the genes which are regulated by the direct interaction of VraR with their promoter regions. The results showed for the first time, that the VraSR mediated regulation of cell wall biosynthesis-associated genes, pbp2, murZ, and sgtB are facilitated by the direct binding of VraR to their respective promoters. Conversely, fmtA, indicated previously to be under VraSR regulation did not exhibit direct regulation by the binding of VraR to its promoter. The VraSR system plays a very important role in antibiotic resistance against cell wall-active antibiotics, and hence, it is essential to understand its complete regulatory mechanism.

References

Mar 11, 2008·The Journal of Biological Chemistry·Antoaneta Belcheva, Dasantila Golemi-Kotra

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Citations

Jan 20, 2016·Microbiology·Justin L BatteMohamed O Elasri
Jun 27, 2018·Journal of Bacteriology·Justin L BatteMohamed O Elasri
Aug 21, 2019·Journal of Bacteriology·Shanti PandeyMohamed O Elasri
Nov 11, 2020·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Neda BaseriBita Bakhshi
Mar 28, 2013·The Journal of Organic Chemistry·Roberta J Worthington, Christian Melander

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