Chromatin organization at the nuclear pore favours HIV replication

Nature Communications
Mickaël LelekFrancesca Di Nunzio

Abstract

The molecular mechanisms that allow HIV to integrate into particular sites of the host genome are poorly understood. Here we tested if the nuclear pore complex (NPC) facilitates the targeting of HIV integration by acting on chromatin topology. We show that the integrity of the nuclear side of the NPC, which is mainly composed of Tpr, is not required for HIV nuclear import, but that Nup153 is essential. Depletion of Tpr markedly reduces HIV infectivity, but not the level of integration. HIV integration sites in Tpr-depleted cells are less associated with marks of active genes, consistent with the state of chromatin proximal to the NPC, as analysed by super-resolution microscopy. LEDGF/p75, which promotes viral integration into active genes, stabilizes Tpr at the nuclear periphery and vice versa. Our data support a model in which HIV nuclear import and integration are concerted steps, and where Tpr maintains a chromatin environment favourable for HIV replication.

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Datasets Mentioned

BETA
PA43002
GSM945267

Methods Mentioned

BETA
super-resolution microscopy
flow cytometry
PCR
light microscopy
electron microscopy
confocal microscopy
nuclear translocation
ELISA
transfection
protein assay

Software Mentioned

ImageJ
STochastic Optical Reconstruction Microscopy ( STORM )
R
LIFTOVER
PALMTT
Matlab
CLC Workbench
STORM

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