Chromatin Remodeling BAF155 Subunit Regulates the Genesis of Basal Progenitors in Developing Cortex

IScience
Ramanathan NarayananTran Tuoc

Abstract

The abundance of basal progenitors (BPs), basal radial glia progenitors (bRGs) and basal intermediate progenitors (bIPs), in primate brain has been correlated to the high degree of cortical folding. Here we examined the role of BAF155, a subunit of the chromatin remodeling BAF complex, in generation of cortical progenitor heterogeneity. The conditional deletion of BAF155 led to diminished bIP pool and increased number of bRGs, due to delamination of apical RGs. We found that BAF155 is required for normal activity of neurogenic transcription factor PAX6, thus controlling the expression of genes that are involved in bIP specification, cell-cell interaction, and establishment of adherens junction. In a PAX6-dependent manner, BAF155 regulates the expression of the CDC42 effector protein CEP4, thereby controlling progenitor delamination. Furthermore, BAF155-dependent chromatin remodeling seems to exert a specific role in the genesis of BPs through the regulation of human RG-specific genes (such as Foxn4) that possibly acquired evolutionary significance.

Citations

May 10, 2019·Wiley Interdisciplinary Reviews. Developmental Biology·Christina Kyrousi, Silvia Cappello
Sep 5, 2019·Frontiers in Cellular Neuroscience·Maxime PenissonFiona Francis
Apr 26, 2019·Annals of Clinical and Translational Neurology·Milka PringsheimKnut Brockmann
May 9, 2021·Neurochemistry International·Leora D'SouzaBhavana Muralidharan
Jun 8, 2021·Frontiers in Cell and Developmental Biology·Godwin SokporTran Tuoc
Oct 20, 2021·PLoS Computational Biology·Eric Ching-Pan ChuPaul Pavlidis
Oct 13, 2021·Human Molecular Genetics·Maxime PenissonRichard Belvindrah

❮ Previous
Next ❯

Methods Mentioned

BETA
delamination
RNA-seq
transgenic
ChIP-seq
bIP
GTPase

Related Concepts

Related Feeds

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.