Chromatin states of core pluripotency-associated genes in pluripotent, multipotent and differentiated cells

Biochemical and Biophysical Research Communications
Sanna Barrand, Philippe Collas

Abstract

Oct4, Nanog and Sox2 constitute a core of transcription factors controlling pluripotency. Differentiation and reprogramming studies have unraveled a few epigenetic modifications associated in relation to the expression state of OCT4, NANOG and SOX2. There is, however, no comprehensive map of chromatin states on these genes in human primary cells at different stages of differentiation. We report here a profile of DNA methylation and of 10 histone modifications on regulatory regions of OCT4, NANOG and SOX2 in embryonal carcinoma cells, mesenchymal stem cells and fibroblasts. Bisulfite sequencing reveals correlation between promoter CpG methylation and repression of OCT4, but not NANOG or SOX2, suggesting distinct repression mechanisms. Whereas none of these genes, even when inactive, harbor repressive trimethylated H3K9, CpG hypomethylated NANOG and SOX2, but not CpG methylated OCT4, are enriched in repressive H3K27me3. H3K79me1 and H3K79me3 tend to parallel each other and are linked to repression. Moreover, we highlight an inverse relationship between H3K27me3 occupancy on promoters and H3K36me3 occupancy on coding regions of OCT4, NANOG and SOX2, suggesting a cross-talk between K27 and K36 methylation. Establishment of distinct...Continue Reading

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Citations

Oct 14, 2011·Cytotechnology·Stefania VioliniPaola Mariani
Nov 2, 2011·Stem Cell Research & Therapy·María Berdasco, Manel Esteller
Oct 6, 2010·Biochemical and Biophysical Research Communications·Sanna BarrandPhilippe Collas
Mar 17, 2010·Trends in Neurosciences·Jia Liu, Patrizia Casaccia
Jul 5, 2012·Journal of Tissue Engineering and Regenerative Medicine·Yanxian PengMichael Raghunath
Jul 15, 2016·Briefings in Functional Genomics·Mònica SuelvesMiguel A Peinado
Nov 8, 2016·Stem Cells International·Monika Glemžaitė, Rūta Navakauskienė
Dec 14, 2018·Cells·Miruna Mihaela MicheuMaria Dorobantu
Sep 4, 2015·Journal of Biosciences·Fathima Athar, Veena K Parnaik
Aug 27, 2015·Molecular and Cellular Biochemistry·Kristina DaniunaiteSonata Jarmalaite
May 11, 2018·Stem Cells International·Hang ZhouYongxiang Wang
Mar 23, 2021·DNA and Cell Biology·Rahyza I F AssisDenise C Andia

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