PMID: 8980667Dec 1, 1996Paper

Chromatin structure and cellular radiosensitivity: a comparison of two human tumour cell lines

International Journal of Radiation Biology
E C WoudstraH H Kampinga

Abstract

The role of variation in susceptibility to DNA damage induction was studied as a determinant for cellular radiosensitivity. Comparison of the radiosensitive HX142 and radioresistant RT112 cell lines previously revealed higher susceptibility to X-ray-induced DNA damage in the sensitive cell line using non-denaturing elution, but not when using alkaline unwinding. The present data also show that no difference in the amount of initial damage is seen when pulsed-field gel electrophoresis (PFGE) or comet analysis are used for DNA damage assessment. However, using the halo assay or a modified version of PFGE in which the higher DNA architecture remained partially intact, the radiosensitive cells showed steeper dose-response curves for initial DNA damage than the radioresistant cells. Analysis of the protein composition, of DNA-nucleoid structures revealed substantial differences when isolated from HX142 or RT112 cells. From our data, it is concluded that HX142 and RT112 differ in their structural organization of chromatin. As no differences in the kinetics of DNA damage rejoining were found, it is hypothesized that the same amount of lesions have a different impact in the two cell lines in that the 'presentation' of DNA damage alters...Continue Reading

References

Oct 1, 1992·International Journal of Radiation Biology·P L Olive
Aug 1, 1992·Cell Biology International Reports·D A JacksonP R Cook
Nov 1, 1991·International Journal of Radiation Oncology, Biology, Physics·J S Bedford
Jun 1, 1990·International Journal of Radiation Biology·J F Ward
Sep 1, 1990·International Journal of Radiation Biology·T J McMillanJ H Peacock
Oct 1, 1989·International Journal of Radiation Biology·D BlöcherJ Zajackowski
Apr 1, 1989·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·M Frankenberg-Schwager
Nov 1, 1980·Cell·B VogelsteinD S Coffey
Jun 16, 1995·Science·Y Baskin
Dec 1, 1994·International Journal of Radiation Biology·W N Hittelman, T K Pandita
Oct 15, 1994·International Journal of Radiation Oncology, Biology, Physics·R WurmJ H Peacock
Nov 1, 1994·International Journal of Radiation Biology·N L OleinickS Chiu
Jul 30, 1994·International Journal of Radiation Oncology, Biology, Physics·S N Powell, T J McMillan
Jan 1, 1993·Environmental and Molecular Mutagenesis·J L Schwartz, A T Vaughan

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Citations

Jul 18, 2003·BMC Cell Biology·Georges NoëlVincent Favaudon
Jan 21, 2006·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Andrei LaszloJoseph L Roti Roti
Jun 19, 2004·International Journal of Radiation Biology·S ChandnaV Jain
Feb 22, 2011·Comptes rendus biologies·Adeline GranzottoNicolas Foray
Mar 13, 2016·Biochimica Et Biophysica Acta·Kanupriya SharmaSudhir Chandna
Dec 14, 2004·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Nicole ChavaudraNicolas Foray
May 9, 2003·The Journal of Veterinary Medical Science·Seiichi WadaNobuhiko Ito
Dec 17, 2003·British Journal of Cancer·M A L MoneefG D D Jones
Aug 23, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Nor F RajabValerie J McKelvey-Martin
Dec 17, 2003·British Journal of Cancer·S R McKeownV J McKelvey-Martin
Mar 10, 2001·Laboratory Investigation; a Journal of Technical Methods and Pathology·C S DjuzenovaM Flentje

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