PMID: 11606254Oct 19, 2001Paper

Chromatin structure at the 3'-boundary of the human beta-globin locus control region hypersensitive site-2

The International Journal of Biochemistry & Cell Biology
A Kim, Vincent Murray

Abstract

Chromatin structure was examined at the 3'-boundary region of the human beta-globin locus control region hypersensitive site-2 (LCR HS-2) using several footprinting agents. Erythroid K562 cells (possessing HS-2) were damaged by the footprinting agents: hedamycin, bleomycin and four nitrogen mustard analogues. Purified DNA and non-erythroid HeLa cells (lacking HS-2) were also damaged as controls for comparison with K562 cells. The comparison between intact cells and purified DNA showed several protected regions in K562 cells. A large erythroid-specific protected region of 135 bp was found at the boundary of HS-2. The length of this protected region (135 bp) was close to that of DNA contained in a nucleosome core (146 bp). Another two protected regions were found upstream of the protected region. A 16-bp erythroid-specific footprint co-localised with a GATA-1 motif-this indicated that the GATA-1 protein could be involved in positioning the nucleosome. Further upstream, a 100-bp footprint coincided with an AT-rich region. Thus our footprinting results suggest that the 3'-boundary of LCR HS-2 is flanked by a positioned nucleosome and that an erythroid-specific protein binds to the sequence adjacent to the nucleosome and acts to pos...Continue Reading

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Jun 17, 2000·The International Journal of Biochemistry & Cell Biology·A Kim, V Murray

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Citations

Oct 31, 2003·Molecular and Cellular Biology·AeRi Kim, Ann Dean
Dec 8, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Long H Chung, Vincent Murray
Dec 25, 2004·The International Journal of Biochemistry & Cell Biology·Mark D Temple, Vincent Murray
Feb 9, 2017·The International Journal of Biochemistry & Cell Biology·Jon K ChenVincent Murray
Jun 23, 2010·Chemical Biology & Drug Design·Anne M Galea, Vincent Murray
Sep 11, 2012·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Hanh T Q Nguyen, Vincent Murray
Jul 16, 2011·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Trung V Nguyen, Vincent Murray
Mar 5, 2016·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Jon K Chen, Vincent Murray
May 18, 2016·Molecular Biology Reports·Vincent MurrayMark M Tanaka
May 8, 2018·International Journal of Molecular Sciences·Vincent MurrayLong H Chung
May 17, 2017·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Shweta D GautamVincent Murray
May 26, 2004·Biochimica Et Biophysica Acta·Mark D TempleVincent Murray
Oct 26, 2016·Bioorganic & Medicinal Chemistry·Jon K ChenVincent Murray

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