PMID: 3763406Sep 25, 1986Paper

Chromatin structure of the promoter region of the human c-K-ras gene

Nucleic Acids Research
J Jordano, M Perucho

Abstract

The chromatin structure of the human c-K-ras gene has been investigated in various cultured normal and tumor human cells and in a rat cell line transformed with the human oncogene. The promoter region is hypersensitive to DNAse I, micrococcal nuclease, endogenous nucleases and to S1 nuclease in supercoiled plasmids. This hypersensitive region is present in the different cell types analyzed and both normal and mutant alleles exhibit similar general sensitivity to DNAse I digestion in the same tumor cells. However, the 5' more distal DNAse I hypersensitive site, which is coincident with a region of the gene containing sequence homologies with known enhancers, exhibits variable sensitivity which appears to be higher in the tumor than in the normal and in the human than in the rat cells which we have analyzed. These data suggest the presence of specific factors interacting with the promoter sequences and delimits the transcription unit of the c-K-ras locus.

References

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Citations

Nov 1, 1990·Molecular Biology Reports·A SinghM S Kanungo
Mar 23, 2011·Chemical Communications : Chem Comm·Manikandan ParamasivamLuigi E Xodo
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T KozasaY Kaziro
Oct 22, 2014·Nucleic Acids Research·Mathieu MétifiotMarie-Line Andreola
Dec 1, 1989·Medical Hypotheses·G Manzo
Jul 26, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Nirali PandyaAmit Kumar

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