Chromatin superstructure-dependent crosslinking with DNA of the histone H5 residues Thr1, His25 and His62

Journal of Molecular Biology
A D MirzabekovK K Ebralidse

Abstract

The points of histone H5 interactions with DNA within nucleosomes and chromatin at different levels of compaction are delineated by identification of H5 amino acid residues that can be covalently bound to DNA. Three major crosslinkable points of H5 are His25, His62 (both within the globular part of the molecule), and N-terminal Thr1. His25 interacts with the terminal regions of nucleosomal DNA; His62 appears to bind more distal segments of the linker DNA. The His25-DNA crosslink predominates in the isolated mononucleosomes and persists throughout the chromatin condensation states studied, from extended oligonucleosomal chains to nuclei. His62 is the strongest crosslinking site in nuclei; in oligonucleosomes, the predominance of the His62-DNA crosslink requires the number of nucleosomes in the chain to be above some critical value. The Thr1-DNA crosslink is generated only in decondensed poly- or oligonucleosomes, but not in mononucleosomes. Thus, underlying the higher-order folding transitions of the nucleosomal chain is the restructuring of H5-DNA interactions.

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M W Hsiang, R D Cole
Jan 25, 1977·Journal of Molecular Biology·M Noll, R D Kornberg
Mar 17, 1975·Biochemical and Biophysical Research Communications·W S Kistler, M E Geroch
Feb 1, 1976·Nucleic Acids Research·A J VarshavskyG P Georgiev
Jan 1, 1989·Methods in Enzymology·A D MirzabekovK K Ebralidse
Apr 25, 1986·Nucleic Acids Research·F Watanabe
Jul 1, 1987·European Journal of Biochemistry·J S Gantt, J L Key
Dec 1, 1988·European Journal of Biochemistry·D J Clark, J O Thomas
Feb 20, 1986·Journal of Molecular Biology·J AllanC Crane-Robinson
Feb 14, 1986·Cell·G Felsenfeld, J D McGhee
Jan 1, 1986·Nucleic Acids Research·D E Wells
Feb 20, 1986·Journal of Molecular Biology·D J Clark, J O Thomas
Jun 5, 1986·Journal of Molecular Biology·W De BernardinT Koller
Jan 28, 1987·Biochimica Et Biophysica Acta·P PuigdomenechC Crane-Robinson
Nov 25, 1985·FEBS Letters·L Böhm, T C Mitchell
Apr 1, 1984·The Journal of Cell Biology·J AllanH Gould
Jul 1, 1984·The Journal of Cell Biology·C L WoodcockJ B Rattner
Jul 24, 1982·Nucleic Acids Research·V L KarpovA D Mirzabekov
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T BoulikasW T Garrard
Aug 25, 1980·Nucleic Acids Research·N M Kumar, I O Walker
May 25, 1980·Journal of Molecular Biology·V V ShickA D Mirzabekov
May 25, 1980·Journal of Molecular Biology·A V BelyavskyA D Mirzabekov
Dec 25, 1980·Nature·J AllanF X Aviles
Jan 1, 1972·Methods in Enzymology·M J Hunter, M L Ludwig

❮ Previous
Next ❯

Citations

Feb 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D PrussA P Wolffe
Apr 1, 1993·Toxicology Letters·T Boulikas
Jun 1, 1991·Current Opinion in Cell Biology·M M Smith
Feb 8, 2006·Nature Structural & Molecular Biology·David T BrownTom Misteli
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·J J HayesA P Wolffe
Feb 2, 2000·The EMBO Journal·A E de la BarreS Dimitrov
Jan 1, 1997·Annual Review of Biophysics and Biomolecular Structure·V Ramakrishnan
May 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Li Fan, Victoria A Roberts
Aug 14, 2013·Biology of the Cell·Jana ŠmigováIvan Raška
Sep 1, 1993·Current Biology : CB·J W Schwabe, A A Travers
Dec 15, 1996·European Journal of Biochemistry·F A GoytisoloJ O Thomas
Oct 1, 1995·Genome Génome / Conseil National De Recherches Canada·J M NeelinK M Cheng
Sep 24, 1998·Journal of Biomolecular Structure & Dynamics·S Belikov, V Karpov
Aug 24, 2013·Proteins·Victoria A RobertsSheng Li
Jun 13, 2001·The Journal of Biological Chemistry·L ScrittoriS Dimitrov
Oct 5, 2001·The Journal of Biological Chemistry·R VilaP Suau
Oct 18, 2020·Nature Communications·Alexandra StützerHenning Urlaub
Sep 28, 2005·Biochemistry·Kohji HizumeKunio Takeyasu

❮ Previous
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