PMID: 6984451Dec 17, 1982Paper

Chromatofocusing as a tool for the characterization and partial purification of human interleukin-2

Journal of Immunological Methods
J P Gérard, J Bertoglio

Abstract

Human interleukin-2 (IL2) has been characterized and partially purified with a sequence of chromatofocusing, gel filtration and SDS-PAGE analysis. IL2 when tested in a [3H]thymidine incorporation assay by human IL2-dependent T cells, appeared to have a MW of 25,000 as determined by Ultrogel ACA 54 gel filtration. Chromatofocusing of an 80% ammonium sulfate precipitate from crude conditioned medium yielded 4 peaks of activity corresponding to fractions of pH 7.65, 7.28, 6.72 and 6.58. Neuraminidase treatment of IL2 prior to chromatofocusing reduced its charge heterogeneity to a single peak of activity at pH 7.63. IL2 which had been treated with neuraminidase, purified by chromatofocusing, radioiodinated and further separated by gel filtration was subjected to SDS gel electrophoresis. We observed a band, migrating in the 15,000 region which only occurred in the active fractions and which we tentatively identified as IL2. These findings indicate that the purification procedure described is appropriate to the characterization and preparation of quantities of human IL2.

References

Dec 28, 1971·Journal of Molecular Biology·J King, U K Laemmli
May 28, 1981·Nature·C S HenneyS Gillis
Jun 1, 1980·The Journal of Experimental Medicine·K A SmithM F Favata
Nov 19, 1981·Nature·R J FordA L Maizel
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J W Mier, R C Gallo
Jan 1, 1980·Journal of Immunological Methods·D Y MochizukiS Gillis
Dec 1, 1981·Molecular Immunology·R J Robb, K A Smith
Jan 1, 1980·Immunological Reviews·K A Smith

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Citations

May 25, 1984·Journal of Immunological Methods·A GodardJ P Soulillou
Jan 1, 1987·International Journal of Immunopharmacology·I C BygbjergK Bendtzen
Sep 6, 2003·Experimental Neurology·Sergueï O FetissovTomas Hökfelt

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