Chromatofocusing fails to separate hFSH isoforms on the basis of glycan structure

Biochemistry
G R BousfieldHeather Desaire

Abstract

Follicle-stimulating hormone (FSH) glycosylation is regulated by feedback from the gonads, resulting in an array of glycans associated with FSH preparations derived from pools of pituitary or urine extracts. FSH glycosylation varies due to inhibition of FSHbeta N-glycosylation, elaboration of 1-4 branches possessed by mature N-glycans, and the number and linkage of terminal sialic acid residues. To characterize FSH glycosylation, FSH isoforms in pituitary gland extracts and a variety of physiological fluids are commonly separated by chromatofocusing. Variations in the ratios of immunological and biological activities in the resulting FSH isoform preparations are generally attributed to changes in glycosylation, which are most often defined in terms of sialic acid content. Using Western blotting to assess human FSHbeta glycosylation inhibition revealed 30-47% nonglycosylated hFSHbeta associated with four of six hFSH isoform preparations derived by chromatofocusing. Glycopeptide mass spectrometry assessment of glycan branching in these isoforms extensively characterized two N-glycosylation sites, one at alphaAsn52, the critical glycan for FSH function, and the other at betaAsn24. With two to four N-glycans per FSH molecule, many ...Continue Reading

References

Jun 1, 1989·Journal of Endocrinological Investigation·W L GordonD N Ward
Apr 1, 1985·Molecular and Cellular Endocrinology·G R BousfieldD N Ward
Dec 1, 1995·Endocrine Reviews·A Ulloa-AguirreV Padmanabhan
Dec 20, 1996·Molecular and Cellular Endocrinology·G R BousfieldW T Moore
Apr 15, 2000·Methods : a Companion to Methods in Enzymology·G R BousfieldV Y Butnev
Apr 18, 2001·Molecular and Cellular Endocrinology·S CreusS Campo
May 4, 2004·Journal of the American Society for Mass Spectrometry·Hui JiangGeorge R Bousfield
Oct 20, 2006·Journal of Chromatography. a·Renan Fernandes LoureiroMaria Teresa C P Ribela

❮ Previous
Next ❯

Citations

Apr 29, 2011·Reviews in Endocrine & Metabolic Disorders·Alfredo Ulloa-AguirreEric Reiter
Jun 28, 2011·Reviews in Endocrine & Metabolic Disorders·Brian J Arey, Francisco J López
Jul 9, 2011·Reviews in Endocrine & Metabolic Disorders·George R Bousfield, James A Dias
Jun 4, 2014·Expert Opinion on Biological Therapy·Daniele Santi, Manuela Simoni
Jan 27, 2009·Journal of Chromatography. a·C M CarvalhoM T C P Ribela
Feb 11, 2015·Molecular and Cellular Endocrinology·Viktor Y ButnevGeorge R Bousfield
Mar 15, 2015·Frontiers in Endocrinology·Claire CahoreauYves Combarnous
Jun 6, 2018·Frontiers in Endocrinology·George R BousfieldT Rajendra Kumar
May 28, 2019·Endocrinology·George R Bousfield, David J Harvey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.