PMID: 6411129Aug 29, 1983Paper

Chromatofocusing of human high density lipoproteins and isolation of lipoproteins A and A-I

Biochimica Et Biophysica Acta
A C NestruckD Seidel

Abstract

Using chromatofocusing, a column chromatography method with an internally generated pH gradient and focusing effects, human plasma high density lipoproteins (HDL) were fractionated into six subclasses within an interval of less than 1 pH unit (pH 5.1-4.2). All fractions floated in the ultracentrifuge at density = 1.21 g X ml-1, retained a typical HDL electron micrographic morphology and as a single band, alpha-migration on agarose electrophoresis. Compositional analysis of the subclasses revealed an inverse relationship between cholesterol ester and cholesterol on a molar basis. Distinct differences in the distribution of the apolipoproteins between the fractions were found. Two of the subclasses contained only apolipoprotein A-I and were therefore considered to be two forms of the lipid-combined form of apolipoprotein A-I, i.e., lipoprotein A-I. One subclass contained only apolipoproteins A-I + A-II and was, therefore, lipoprotein A. One subclass contained apolipoproteins A-I + A-II + D, and the two remaining contained additionally apolipoproteins C and E. Lipoprotein A-I was also demonstrated after immunoabsorption of apolipoprotein A-II-containing lipoproteins from whole serum. It is suggested that this method, which allows ...Continue Reading

References

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Citations

Sep 1, 1991·Diabetes/metabolism Reviews·C Fievet, J C Fruchart
Jun 6, 1998·Clinica Chimica Acta; International Journal of Clinical Chemistry·A AdlouniA Benslimane
Nov 16, 2010·Biochemistry·Kekulawalage GauthamadasaR A Gangani D Silva
Jun 3, 1999·European Journal of Clinical Investigation·F GottrandG Luc
Feb 1, 1991·European Journal of Clinical Investigation·G LucJ C Fruchart
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Nov 1, 1987·Atherosclerosis·P PuchoisJ C Fruchart
Jun 1, 1996·Critical Reviews in Clinical Laboratory Sciences·A Tailleux, J C Fruchart
May 10, 2017·Journal of Lipid Research·John T MelchiorW Sean Davidson
Dec 1, 2017·Lipids in Health and Disease·Toshimi KidoShinji Yokoyama
Jan 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·T OhtaI Matsuda
Sep 1, 1988·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·W MärzW Gross
Dec 1, 1991·Metabolism: Clinical and Experimental·G LucJ Davignon

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