CHROMATOGRAPHIC ANALYSIS OF DRUG INTERACTIONS IN THE SERUM PROTEOME

Analytical Methods : Advancing Methods and Applications
D S HageXiwei Zheng

Abstract

The binding of drugs with serum proteins and binding agents such as human serum albumin, α1-acid glycoprotein, and lipoproteins is an important process in determining the activity and fate of many pharmaceuticals in the body. A variety of techniques have been used to study drug interactions with serum proteins, but there is still a need for faster or better methods for such work. High-performance liquid chromatography (HPLC) is one tool that has been utilized in many formats for these types of measurements. Advantages of using HPLC for this application include its speed and precision, its ability to be automated, its good limits of detection, and its compatibility with a wide range of assay formats and detectors. This review will discuss various approaches in which HPLC can be employed for the study of drug-protein interactions. These techniques include the use of soluble proteins in zonal elution and frontal analysis methods or vacancy techniques such as the Hummel-Dreyer method. Zonal elution and frontal analysis methods that make use of immobilized proteins and high-performance affinity chromatography will also be presented. A variety of applications will be examined, ranging from the determination of free drug fractions to ...Continue Reading

References

Dec 11, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ryan MatsudaD S Hage
Feb 7, 2012·Journal of Pharmaceutical and Biomedical Analysis·D S HageXiwei Zheng
Jan 3, 2016·Drug Discovery Today. Technologies·Cong BiD S Hage
Dec 3, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Cong BiD S Hage
Feb 1, 2017·The Journal of General Virology·Boghuma Kabisen TitanjiClare Jolly
Jun 28, 2012·Analytical Methods : Advancing Methods and Applications·Lin-Sen QingXun Liao

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Related Concepts

Study
Protein Binding
High Throughput Screening
Glycoproteins
Chromatography
Determination Aspects
ALB
Albumin Human, USP
Human Serum Albumin [EPC]
Serum Proteins

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