Chromatographic and immunological evidence that chloroplastic and cytosolic pea (Pisum sativum L.) NADP-isocitrate dehydrogenases are distinct isoenzymes

Planta
R D ChenP Gadal

Abstract

Two NADP-isocitrate dehydrogenase isoenzymes designated as NADP-IDH1 and NADP-IDH2 (EC 1.1.1.42) were identified in pea (Pisum sativum) leaf extracts by diethylaminoethylcellulose chromatography. The predominant form was found to be NADP-IDH1 while NADP-IDH2 represented only about 4% of the total leaf enzyme activity. These enzymes share few common epitopes as NADP-IDH2 was poorly recognized by the specific polyclonal antibodies raised against NADP-IDH1, and as a consequence NADP-IDH2 does not result from a post-translational modification of NADP-IDH1. Subcellular fractionation and isolation of chloroplasts through a Percoll gradient, followed by the identification of the associated enzymes, showed that NADP-IDH1 is restricted to the cytosol and NADP-IDH2 to the chloroplasts. Compared with the cytosolic isoenzyme, NADP-IDH2 was more thermolabile and exhibited a lower optimum pH. The data reported in this paper constitute the first report that the chloroplastic NADP-IDH and the cytosolic NADP-IDH are two distinct isoenzymes. The possible functions of the two isoenzymes are discussed.

References

Sep 14, 1977·Biochimica Et Biophysica Acta·H Y Nakatani, J Barber
Jan 18, 1973·Biochimica Et Biophysica Acta·R DouceW D Bonner
Sep 1, 1983·Journal of Biochemistry·K Kobayashi, S Tuboi

Citations

Jun 19, 2001·The International Journal of Biochemistry & Cell Biology·J B BarrosoJ A Lupiáñez
May 1, 1996·Journal of Cardiovascular Pharmacology·L ChenS S Lee
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·S GálvezM Hodges
Jun 19, 1998·Biochimica Et Biophysica Acta·T N Popova, M A Pinheiro de Carvalho

Related Concepts

Epitopes
Chloroplasts
Chromatography
Isoenzymes
Isocitrate dehydrogenase (NADP+)
Isolation Aspects
Plant Leaves
Polyclonal antibody
Pisum sativum
Cytoplasmic Matrix

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