Chromatographic and immunological identification of GnRH (gonadotropin-releasing hormone) variants. Occurrence of mammalian and a salmon-like GnRH in the forebrain of an eutherian mammal: Hydrochaeris hydrochaeris (Mammalia, Rodentia)

Regulatory Peptides
A D MontanerJ M Affanni

Abstract

The molecular variants of Gonadotropin releasing hormone (GnRH) in brain extracts of the eutherian mammal Hydrochaeris hydrochaeris (Mammalia, Rodentia) were characterized. An indirect method combining reverse-phase high-performance liquid chromatography (RP-HPLC) and radioimmunoassay (RIA) with different antisera was used. Two different forebrain regions (olfactory bulbs and preoptic-hypothalamic region) were analyzed. Characterization of RP-HPLC fractions from preoptic-hypothalamic extracts with three different RIA systems revealed two immunoreactive GnRH (ir-GnRH) peaks coeluting with mammalian GnRH (mGnRH) and salmon GnRH (sGnRH) synthetic standards. These results were additionally supported by serial dilution studies with specific antisera. Similar results were obtained from olfactory bulb extracts with the same methodology. However, a third ir-GnRH peak in a similar position to that of chicken GnRH II (cIIGnRH) synthetic standard was revealed. As far as we know, this is the first report showing chromatographic and immunological evidences for the presence of a second GnRH variant in the forebrain of an eutherian mammal.

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Citations

Apr 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ishwar S ParharYasuo Sakuma
Sep 28, 2000·Biochemical and Biophysical Research Communications·K OkuboK Aida
Aug 11, 2000·General and Comparative Endocrinology·A D MontanerG M Somoza
Jun 8, 2012·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Yong-Jiang XuQing-Yin Wang
Jun 17, 2006·Molecular and Cellular Endocrinology·K E WhitlockS Twomey
Oct 14, 2014·Frontiers in Neuroendocrinology·Paolo E Forni, Susan Wray
Dec 20, 2007·Reproductive Sciences·Chryssa MetallinouNikos Nikolettos
Feb 28, 2002·Endocrinology·Jimmy D Neill
Jan 1, 2006·Expert Review of Endocrinology & Metabolism·Alexander S Kauffman, Emilie F Rissman
May 24, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Leonardo G GuilgurGustavo M Somoza

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