Chromatographic characterization of phosphonate analog EDTA-modified zirconia support for biochromatographic applications

Analytical Chemistry
A M Clausen, P W Carr

Abstract

Zirconium dioxide (zirconia) has a great affinity for inorganic and organic phosphate. Previous work from this laboratory demonstrated the utility of phosphate-modified microparticulate zirconia as a support for protein separations. We have extended this investigation to include the study of ethylenediamine-N,N'-tetramethylphosphonic acid (EDTPA), a phosphonate analog of EDTA, as a surface modifier for zirconia. Our work explores the use of EDTPA-modified zirconia (PEZ) for its potential use as a high-performance inorganic cation-exchange support for the separation of proteins. The phosphate groups in EDTPA very effectively block the sites responsible for strong interactions of hard Lewis bases with zirconia's surface. Modification of zirconia with EDTPA provides a "biocompatible" stationary phase, resulting in high mass recoveries of proteins. We compare PEZ with inorganic phosphate-modified zirconia to show increased efficiency, as well as unique selectivities for chromatography of proteins on the chelator-modified surface. Finally, the selectivity, efficiency, and separation mechanism are reported. The studies show that PEZ is a useful high-performance ion-exchange support for the separation of cationic proteins and for modu...Continue Reading

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Citations

Apr 10, 2013·Acta Biomaterialia·Laura TreccaniKurosch Rezwan
Apr 26, 2012·Chemical Reviews·Clémence QueffélecBruno Bujoli
Oct 14, 2009·Journal of Separation Science·Yun Li, Milton L Lee
Apr 16, 2002·Journal of the American Society for Mass Spectrometry·Wing Lam, Ragu Ramanathan
Jan 18, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Jung-Min OhStephen E Creager
Nov 16, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Francesca BellezzaMaria Anna Quotadamo
Jul 17, 1999·Analytical Chemistry·C K LariveS Bogdanowich-Knipp
May 7, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·David CarrièreOlivier Spalla

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