Chromatographic forms of terminal deoxynucleotidyl transferase in normal lymphoid cells and in leukemia cells at presentation and relapse

Leukemia Research
R BellR P McCaffrey

Abstract

Normal thymocyte and bone marrow terminal deoxynucleotidyl transferase (TdT) have distinguishing characteristics by phosphocellulose chromatography in Tris buffer: marrow TdT elutes as a single peak at 0.3 M salt, whereas thymocyte TdT separates into two forms, one at 0.3 M salt and one at 0.4 M salt. Since the majority of TdT-positive acute leukemias are anatomically bone marrow-derived, one would have predicted the presence of a bone marrow TdT-phosphocellulose chromatographic pattern in such patients. However, in 376 consecutive, untreated TdT-positive acute lymphoblastic leukemias (ALL) studied by us we have invariably encountered the two-peak thymocyte-type phosphocellulose pattern. The TdT patterns in the thymic-dependent, TdT-positive lymphoma of AKR mice, and the TdT-positive bone marrow-derived, thymic-independent Abelson virus leukemia of Balb/C mice duplicate the situation in human ALL: a thymocyte pattern is seen in both the marrow-derived and thymus-derived diseases. This chromatographic difference between leukemia-associated and normal marrow-associated TdT in both murine and human leukemia suggested that phosphocellulose-TdT patterns might be useful for monitoring residual marrow tumour cell burden in TdT-positiv...Continue Reading

References

Jan 1, 1977·Cold Spring Harbor Symposia on Quantitative Biology·D BaltimoreR P McCaffrey
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Apr 10, 1975·The New England Journal of Medicine·R McCaffreyD Baltimore
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Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R McCaffreyD Baltimore

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Citations

Nov 1, 1983·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·R DaculsiJ F Duplan

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