Chromatographic method for the simultaneous quantification of dapsone and clofazimine in nanoformulations

Journal of Separation Science
Sandia MachadoMarcela A Segundo

Abstract

The low bioavailability and nonspecific distribution of dapsone and clofazimine, commonly applied in combination for the treatment of leprosy, can produce toxic effects. Nanotechnological approaches enhance the delivery of these drugs. Therefore, a high-performance liquid chromatography method was developed for the simultaneous determination of dapsone and clofazimine loaded in nanoformulations for quality control purposes. Chromatographic separation was achieved on a reversed-phase Kinetex core-shell C18 column, followed by spectrophotometric detection at 280 nm. Considering the different physicochemical properties of dapsone and clofazimine, elution was performed in gradient mode using an aqueous acetate buffer (50 mmol/L, pH 4.8) and an increasing acetonitrile content from 27 to 63% v/v at a flow rate of 1.0 mL/min with retention times of 6.2 and 14.0 min, respectively. The method was validated according to the European Medicines Agency guideline and it was found to be specific, accurate (99.6-114.0%), and precise for intra- (RSD ≤ 1.8%) and interday assays (RSD ≤ 12.5%). Both drugs showed stability after 24 h at room temperature and over three freeze-thaw cycles with recoveries ≥86.2%. Low temperature (4°C) in the autosampl...Continue Reading

References

Jun 7, 1996·Journal of Chromatography. B, Biomedical Applications·R O'ConnorR O'Kennedy
May 30, 2001·Journal of Pharmaceutical and Biomedical Analysis·H D Revanasiddappa, B Manju
May 21, 2011·International Journal of Pharmaceutics·Khalil MitriRainer H Müller
Oct 25, 2011·The Journal of Antimicrobial Chemotherapy·Moloko C CholoRonald Anderson
Oct 28, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Eiichi YamamotoNaoki Asakawa
Aug 9, 2013·Expert Opinion on Drug Discovery·Shanmugam Anusuya, Jeyakumar Natarajan
Dec 7, 2013·Archives of Dermatological Research·Gottfried Wozel, Christian Blasum
Jun 8, 2014·Bioorganic & Medicinal Chemistry Letters·Paulo Renato YamasakiJean Leandro dos Santos
Sep 1, 2015·International Journal of Biological Macromolecules·Luíse L ChavesSalette Reis
Sep 1, 2015·Acta Biomaterialia·Luis RojoJulio San Roman
Oct 8, 2016·Journal of Separation Science·Qian ZhangZhi-Ning Xia
Jul 12, 2017·The Lancet Infectious Diseases·Cairns S SmithMarcos Virmond
Sep 21, 2017·Molecular Pharmaceutics·Yingyue ZhangRobert K Prud'homme
Nov 11, 2017·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Luíse L ChavesSalette Reis
Jun 6, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Luíse L ChavesSalette Reis

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Citations

Jul 11, 2020·Combinatorial Chemistry & High Throughput Screening·Sercan YıldırımAhmet Yaşar

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