Chromatographic performance of synthetic polycrystalline diamond as a stationary phase in normal phase high performance liquid chromatography

Journal of Chromatography. a
Anton PeristyyP N Nesterenko

Abstract

The chromatographic properties of high pressure high temperature synthesised diamond (HPHT) are investigated in normal phase mode of high performance liquid chromatography. Purified nonporous irregular shape particles of average particles size 1.2 μm and specific surface area 5.1 m(2) g(-1) were used for packing 100×4.6 mm ID or 50×4.6 mm ID stainless steel columns. The retention behaviour of several classes of compounds including alkyl benzenes, polyaromatic hydrocarbons (PAH), alkylphenylketones, phenols, aromatic acids and bases were studied using n-hexane-2-propanol mixtures as mobile phase. The results are compared with those observed for microdispersed sintered detonation nanodiamond (MSDN) and porous graphitic carbon (PGC). HPHT diamond revealed distinctive separation selectivity, which is orthogonal to that observed for porous graphitic carbon; while selectivities of HPHT diamond and microdispersed sintered detonation nanodiamonds are similar. Owing to non-porous particle nature, columns packed with high pressure high temperature diamond exhibited excellent mass transfer and produce separations with maximum column efficiency of 128,200 theoretical plates per meter.

References

Mar 7, 2007·Journal of Chromatography. a·Pavel N NesterenkoPhil Jones
May 29, 2008·Doklady. Biochemistry and Biophysics·K V PurtovV S Bondar
Jul 26, 2008·Proteomics·Johannes StadlmannFriedrich Altmann
Oct 17, 2008·Journal of Chromatography. a·Grace W MunaMarc D Porter
Jun 9, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jun WangM S Yang
Oct 9, 2009·Journal of Chromatography. a·C WestM Lafosse
Nov 3, 2009·Analytical and Bioanalytical Chemistry·Pavel N Nesterenko, Paul R Haddad
Aug 31, 2010·Journal of Chromatography. a·Michael MelmerWolfgang Lindner
Apr 26, 2013·Journal of Separation Science·Rajesh ChittaWillem deGroot
Jul 8, 2014·Journal of Chromatography. a·Anton A PeristyyPavel N Nesterenko

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Citations

Jan 21, 2016·ACS Applied Materials & Interfaces·Zuqin XueLuis A Colon
Dec 23, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·René Holm, David P Elder
Oct 16, 2020·Journal of Separation Science·Mimi J den UijlMaarten R van Bommel

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