Chromatographic purification of recombinant human erythropoietin

Biotechnology Letters
Jana AdamíkováMilan Polakovič

Abstract

Recombinant human erythropoietin is a valuable therapeutic protein used in the treatment of several serious diseases. It exists in different isoforms and is produced by genetically modified mammalian cells such as Chinese hamster ovary or human embryonic kidney cells. As for other biopharmaceutical drugs, a key factor for its successful industrial production is to achieve a high degree of purity and to decrease the content of critical impurities to trace amounts. This goal is achieved in the separation sequence which substantial part is formed by chromatographic steps. Therefore, downstream processing forms an essential part of production costs. This review presents the overview of published separation sequences and, analyzes the use of different types of chromatographic media such as affinity, ion-exchange, reversed-phase, hydrophobic interaction, multimodal, and size-exclusion chromatography adsorbents. Their application is discussed with regard to their place in the purification stages generally denoted as capture, intermediate purification and polishing.

References

Dec 1, 1992·Biologicals : Journal of the International Association of Biological Standardization·T HayakawaM Ueda
Sep 1, 1990·British Journal of Haematology·L Wide, C Bengtsson
Sep 1, 1988·Archives of Biochemistry and Biophysics·V C BroudyJ S Powell
Jun 5, 1987·Journal of Chromatography·H YanagiS Ogino
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F K LinZ Stabinsky
Jan 1, 1985·The International Journal of Biochemistry·F J Joubert, E H Merrifield
Aug 1, 1972·Canadian Journal of Biochemistry·W A Lukowsky, R H Painter
Apr 20, 2001·British Journal of Cancer·J C Egrie, J K Browne
Nov 13, 2003·British Journal of Cancer·J W LeeS H Lee
May 4, 2004·Methods : a Companion to Methods in Enzymology·Andrea Amalfitano
Jul 24, 2004·Biotechnology and Applied Biochemistry·Yunlong HuShuangquan Zhang
Sep 11, 2004·Protein Expression and Purification·Peter Astrup ChristensenSteffen Goletz
Feb 10, 2007·Current Medical Research and Opinion·Jonathan T C KwanUNKNOWN Epoetin Delta Study Group
Jun 28, 2007·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Wolfgang Jelkmann
Mar 25, 2008·Protein Expression and Purification·Silke GerberMarkus Pietzsch
Apr 4, 2008·Annals of Oncology : Official Journal of the European Society for Medical Oncology·T M KimUNKNOWN Lymphoma Subcommittee of the Korean Cancer Study Group
Dec 23, 2008·Journal of Proteome Research·David C A NevilleTerry D Butters
Nov 7, 2009·Methods in Enzymology·Larry J CummingsKimberly Brisack
Jan 5, 2011·Drug Testing and Analysis·Christian ReichelFriedrich Abzieher
Apr 19, 2011·ACS Combinatorial Science·María C Martínez-CeronSilvia A Camperi
Jun 4, 2011·Molecular Immunology·Mamta ShuklaBhola Nath Paul
Sep 3, 2011·Indian Journal of Nephrology·P MalleshappaA B Shah
Sep 27, 2012·Angewandte Chemie·Ping WangSamuel J Danishefsky
Mar 5, 2014·Journal of Molecular Recognition : JMR·Tiago MatosLeif Bülow
Jun 25, 2014·The American Journal of Gastroenterology·Michael S EpsteinUNKNOWN FDA-Related Matters Committee of the American College of Gastroenterology
Apr 7, 2018·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·William S KishRuben G Carbonell

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
suitable
glycosylation
size-exclusion chromatography
ELISA
reversed phase chromatography

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the Society for Experimental Biology and Medicine
W A RAMBACHH L ALT
Current Protein & Peptide Science
Vivek HalanAnurag S Rathore
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
Takeshi Omasa
Current Protocols in Protein Science
A Williams
© 2022 Meta ULC. All rights reserved