Chromatographic resolution, characterisation and quantification of VX enantiomers in hemolysed swine blood samples

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
G ReiterF Worek

Abstract

The present study was initiated to develop a sensitive and highly selective method for the analysis of the enantiomers of the nerve agent VX (O-ethyl S-[2(diisopropylamino)ethyl] methylphosphonothioate) in blood samples for toxicokinetic and therapeutic research. To achieve this goal, analytical and semi-preparative enantioseparation of VX were carried out with gas and liquid chromatography. The GC chiral stationary phase was HYDRODEX-beta-TBDAc (beta cyclodextrin), on which VX was baseline-resolved. On the chiral HPLC phase CHIRALCEL OD-H the enantiomers of VX were isolated with enantiomeric excess >99.99%. They were characterised by specific optical rotation (+/-25.8 deg ml dm(-1)g(-1) at 20 degrees C and 589 nm) and by determination of cholinesterase inhibition rate constants. For the quantitative chiral detection of VX the enantioresolution was realized on the HPLC chiral phase CHIRAL AGP. A specific procedure was developed to isolate VX from swine blood samples thereby stabilising its enantiomers. The limit of detection was 200 fg per enantiomer on column. The absolute recovery of the overall sample preparation procedure was 75%. After an intravenous and percutaneous administration of a supralethal dose of VX in anesthetis...Continue Reading

Citations

Sep 24, 2013·Archives of Toxicology·Timo WilleFranz Worek
Jul 23, 2015·Toxicology Letters·Franz WorekTimo Wille
Nov 3, 2010·Toxicology Letters·Susanne MüllerGeorg Reiter
Dec 8, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T WilleF Worek
Mar 20, 2014·Toxicology Letters·Georg ReiterFranz Worek
Jun 29, 2016·Archives of Toxicology·Franz WorekHorst Thiermann
May 3, 2011·Toxicology Letters·T W SawyerS Bohnert
Apr 14, 2017·Bioorganic & Medicinal Chemistry·Ahmed MohammedCynthia M Dupureur
Jul 13, 2019·International Journal of Legal Medicine·Sofia SoaresEugenia Gallardo
Apr 2, 2011·The Journal of Biological Chemistry·Marielle WandhammerFlorian Nachon
Oct 12, 2019·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·Timo WilleHorst Thiermann
May 5, 2016·Chemico-biological Interactions·Helen RiceJohn E H Tattersall
May 26, 2010·Analytical Chemistry·Timothy J Ward, Karen D Ward
Jul 18, 2021·Toxicology and Applied Pharmacology·Alex S CornelissenMarloes J A Joosen
Dec 29, 2021·Archives of Toxicology·Lisa StiglerTimo Wille

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