PMID: 8454703Jan 29, 1993Paper

Chromatographic separation of glycated nucleotides

Journal of Chromatography
I MiksíkZ Deyl

Abstract

A high-performance liquid chromatographic method for the separation of nucleotide glycation reaction product(s) has been developed. The product(s) arising during in vitro glycation of a nucleotide (AMP, GMP or CMP) with a reducing sugar (ribose or glucose) were clearly resolved from the non-glycated constituents of the reaction mixture. The peak(s) of the glycated product(s) increased when an amino acid (lysine, arginine, beta-alanine or N epsilon-acetyllysine) was added to the reaction mixture. This increase probably corresponds to the formation of a new product with a different absorption maximum (250 versus 260 nm). Conversely, formation of this product(s) was inhibited by the presence of the metal-chelating agent diethylenetriamine pentaacetic acid and by aminoguanidine.

References

Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A T Lee, A Cerami
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R BucalaA Cerami
Mar 5, 1971·Science·G B PriceT Makinodan
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R BucalaA Cerami

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Citations

Oct 8, 1999·Rapid Communications in Mass Spectrometry : RCM·A LapollaP Traldi

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