PMID: 9446840Mar 7, 1998Paper

Chromium picolinate modulates rat vascular smooth muscle cell intracellular calcium metabolism

The Journal of Nutrition
J W MooreM B Zemel

Abstract

We have previously shown that insulin attenuates vasoconstriction, accelerates both vascular relaxation and [Ca2+]i recovery from pressor agonist-induced Ca2+ loads, and stimulates Ca2+-ATPase gene expression in rat and human vascular smooth muscle cells (VSMC). Moreover, these functions are impaired in VSMC from both insulin resistant and insulinopenic rats, suggesting that hypertension in insulin resistant states may result, in part, from impaired insulin-regulation of VSMC Ca2+ transport. Accordingly, we have now evaluated the effect of improving cellular insulin sensitivity with chromium picolinate (CrPic) on regulation of VSMC Ca2+ transport. Cultured VSMC from rats were grown from passage to confluence in the presence or absence of 1 micromol/L CrPic, maintained in a quiescent medium for 24 h and incubated with or without insulin (10(-8) mol/L) for the final 2 h. Cells were then harvested and RNA and protein extracted for Northern and Western blot analysis, respectively. Insulin caused a significant stimulation of plasmalemmal Ca2+-ATPase mRNA and protein (P < 0.05). A comparable stimulation of the mRNA and protein levels was caused by CrPic in the absence of insulin (P < 0.05), while the CrPic + insulin treatment caused ...Continue Reading

References

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Jun 1, 1991·American Journal of Hypertension·M B ZemelJ R Sowers
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Citations

Nov 24, 1999·Current Opinion in Clinical Nutrition and Metabolic Care·H G Preuss, R A Anderson
Jun 21, 2003·The American Journal of Clinical Nutrition·Mark F McCarty
Jul 19, 2008·Molecular and Cellular Biochemistry·Francis C LauDebasis Bagchi

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