Chromium VI-induced apoptosis in a human bronchial epithelial cell line (BEAS-2B) and a lymphoblastic leukemia cell line (MOLT-4)

Journal of Occupational and Environmental Medicine
Angela GambelungheGiacomo Muzi

Abstract

Hexavalent chromium compounds are well-documented human carcinogens. In vitro experiments show Cr (VI) induces cell death by apoptosis by activating p53 protein. The aim of this study was to evaluate Cr (VI)-induced apoptosis in a human bronchial epithelial cell line (BEAS-2B) and in a lymphoblastic leukemia cell line (MOLT-4). Cr (VI) caused a dose- and time-dependent increase in the apoptosis rate in both cell lines. Western blotting showed increased p53 protein expression in MOLT-4 cells, but not in BEAS-2B cells, after exposure to 0.5 and 3 muM hexavalent chromium for 12 hours and 4 hours, respectively. Apoptotic cell death induced by Cr (VI) was not decreased by pretreatment with caspase-3, -8, and -9 inhibitors. These preliminary results provide evidence of Cr (VI)-induced apoptosis, which deserves further investigation in occupationally exposed workers.

References

Jul 1, 1989·Biological Trace Element Research·S De FloraP Zanacchi
Aug 30, 1989·Biochemical and Biophysical Research Communications·X L Shi, N S Dalal
Jan 1, 1980·International Review of Cytology·A H WyllieA R Currie
May 1, 1994·Toxicology and Applied Pharmacology·L J BlankenshipS R Patierno
Jul 1, 1997·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·K B WallaceD P Jones
Feb 7, 1998·The Journal of Biological Chemistry·M L AgarwalG R Stark
Jul 4, 1998·Journal of Toxicology and Environmental Health. Part a·H ShimadaM P Waalkes
Aug 28, 1998·Science·N A Thornberry, Y Lazebnik
Mar 19, 1999·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·X ShiV Vallyathan
Nov 24, 1999·Toxicology and Applied Pharmacology·A Flores, J M Pérez
Nov 27, 1999·The Journal of Biological Chemistry·J YeX Shi
May 2, 2000·Toxicological Sciences : an Official Journal of the Society of Toxicology·D L CarlisleS R Patierno
May 9, 2000·Toxicology and Applied Pharmacology·C YuanM P Waalkes
Jun 29, 2000·Annual Review of Biochemistry·W C EarnshawS H Kaufmann
Jul 20, 2000·Molecular Carcinogenesis·D L CarlisleS R Patierno
Jul 27, 2000·Toxicological Sciences : an Official Journal of the Society of Toxicology·M P WaalkesM J McCabe
Aug 16, 2000·American Journal of Physiology. Cell Physiology·S WangX Shi
Aug 2, 2001·Biochemical and Biophysical Research Communications·C VasantT Ramasami
Aug 9, 2001·The Journal of Biological Chemistry·S J CharetteJ Landry
Mar 30, 2002·Critical Reviews in Oncology/hematology·Fei Chen, Xianglin Shi
Sep 27, 2002·Molecular Carcinogenesis·Alberto IzzottiSilvio De Flora

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Citations

Nov 15, 2011·Toxicology and Applied Pharmacology·Fen WuMax Costa
Aug 5, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Yoshiko Koike-KurodaShinji Tsukahara
Feb 27, 2007·Free Radical Biology & Medicine·Griselda R BorthiryCharles R Myers
May 1, 2012·Free Radical Biology & Medicine·Charles R Myers
Jun 21, 2011·Journal of Occupational and Environmental Medicine·Angela GambelungheGiacomo Muzi

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