Chromium(III) Binding Phage Screening for the Selective Adsorption of Cr(III) and Chromium Speciation

ACS Applied Materials & Interfaces
Ting YangJian-Hua Wang

Abstract

The screening of suitable sorption medium is the key for highly selective solid phase extraction (SPE) of heavy metals. Herein, we demonstrate a universal protocol for producing selective SPE adsorbent through an evolutional approach based on phage display peptide library. By choosing chromium(III) as the model target, immobilized Cr(III) resins are first prepared using Ni-NTA affinity resins for the interaction with NEB heptapeptide phage library. After three rounds of positive biopanning against target Cr(III) and negative biopanning against foreign metal species, Cr(III) binding phages with high selectivity are obtained. The binding affinity and selectivity are further assessed with ELISA. The phages bearing peptide (YKASLIT) is finally chosen and immobilized on cytopore beads for Cr(III) preconcentration. The retained Cr(III) is efficiently recovered by 0.10 mol L(-1) HNO3 and quantified with ICP-MS. By loading 4000 μL of sample solution at pH 7.0 for 2 h and stripping with 400 μL of 0.10 mol L(-1) HNO3, a linear range of 0.05-0.50 μg L(-1) is achieved along with an enrichment factor of 7.1. The limit of detection is derived to be 15 ng L(-1) (3σ, n = 7) with a RSD of 3.6% (0.25 μg L(-1), n = 7). The procedure is validated ...Continue Reading

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Citations

Mar 18, 2020·Critical Reviews in Analytical Chemistry·Yi-Kun LiJian-Hua Wang
May 21, 2019·Biomimetics·Nora SchönbergerKatrin Pollmann
May 10, 2020·The Science of the Total Environment·Prakriti Sharma GhimireShichang Kang
Feb 27, 2021·Journal of Hazardous Materials·Nora SchönbergerKatrin Pollmann
Jul 3, 2021·International Journal of Molecular Sciences·Xiaoyu ZhangHuiling Gao

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