Chromodomain helicase DNA binding protein 5 plays a tumor suppressor role in human breast cancer.

Breast Cancer Research : BCR
Xiao WuJin-Tang Dong

Abstract

The chromodomain helicase DNA binding protein 5 (CHD5) has recently been identified as a tumor suppressor in a mouse model. The CHD5 locus at 1p36 is deleted, and its mutation has been detected in breast cancer. We, therefore, evaluated whether CHD5 plays a role in human breast cancer. We screened mutations in 55 tumors, determined promoter methylation in 39 tumors, measured RNA expression in 90 tumors, analyzed protein expression in 289 tumors, and correlated expression changes with clinicopathological characteristics of breast cancer. Functional effects of CHD5 on cell proliferation, invasion and tumorigenesis were also tested. Although only one mutation was detected, CHD5 mRNA expression was significantly reduced, accompanied by frequent genomic deletion and promoter methylation, in breast cancer. The extent of methylation was significantly associated with reduced mRNA expression, and demethylating treatment restored CHD5 expression. Lower CHD5 mRNA levels correlated with lymph node metastasis (P = 0.026). CHD5 protein expression was also reduced in breast cancer, and lack of CHD5 expression significantly correlated with higher tumor stage, ER/PR-negativity, HER2 positivity, distant metastasis and worse patient survival (P ≤...Continue Reading

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Aug 21, 2013·Mutation Research·Fintan K T StanleyAaron A Goodarzi
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Jul 27, 2021·Frontiers in Molecular Neuroscience·Yue QiJun Wan

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Methods Mentioned

BETA
PCR
transfection

Software Mentioned

SPSS

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