Chromogenic detection of aminoglycoside phosphotransferases.

Antimicrobial Agents and Chemotherapy
A M Amoroso, G O Gutkind

Abstract

A coupled chromogenic reaction (based on an agar overlay combining NADH, pyruvate kinase, lactate dehydrogenase, phosphoenolpyruvate, ATP, and kanamycin sulfate with thiazolyl blue-phenazine methosulfate for detection of NADH consumption) was optimized for the detection of aminoglycoside phosphotransferases (APHs). When used after analytical isoelectrofocusing of bacterial extracts from APH-producing strains, this method revealed one band in each of two strains with a genetically confirmed APH (3') I and two bands in another strain with both APH (3') I and APH (3') VI, whereas no bands were detected in susceptible control strains or in aminoglycoside-resistant microorganisms without APH genes.

References

Mar 8, 1976·Biochemical and Biophysical Research Communications·P R Goldman, D B Northrop
Apr 1, 1970·Biochemical Genetics·C R Shaw, R Prasad

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Citations

Mar 6, 1999·Current Opinion in Microbiology·K Bush, G H Miller
Dec 8, 2004·Experimental Parasitology·Marlei J AugustoLuiz R O Tosi

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