Chromokinesin Kid and kinetochore kinesin CENP-E differentially support chromosome congression without end-on attachment to microtubules

Nature Communications
Kenji Iemura, Kozo Tanaka

Abstract

Chromosome congression is the alignment of chromosomes at the spindle equator, and is a prerequisite for faithful chromosome segregation. Recent data suggest that before kinetochores attach to the end of microtubules (end-on attachment), chromosomes can move along microtubules towards the spindle equator through attachment of kinetochores to the lateral surface of microtubules (lateral attachment). Here we address this mechanism, focusing on the contribution of two mitotic motors, Kid and CENP-E. In cells depleted of Hec1, which is essential for end-on attachment, chromosomes show partial and transient congression. This transient congression is further perturbed by co-depletion of Kid, suggesting its role in chromosome congression. In comparison, CENP-E suppresses chromosome congression, probably by tethering kinetochores to short, unstable microtubules, and works in congression only when microtubules are stabilized. Our results may reflect the differential contributions of Kid and CENP-E in chromosome congression in physiological conditions where stabilized microtubules are becoming increased.

References

Nov 1, 1995·The Journal of Cell Biology·Y ZhaiG G Borisy
Feb 12, 1998·The Journal of Cell Biology·B T SchaarT J Yen
Aug 15, 1998·Trends in Cell Biology·C L Rieder, E D Salmon
Dec 12, 2001·Molecular Biology of the Cell·H J MatthiesR S Hawley
Feb 28, 2003·The EMBO Journal·Junichiro YajimaYoko Y Toyoshima
Jul 9, 2004·Developmental Cell·Patrick MeraldiPeter K Sorger
Apr 23, 2005·Nature·Kozo TanakaTomoyuki U Tanaka
Sep 24, 2005·Molecular Biology of the Cell·Noriko Tokai-NishizumiTadashi Yamamoto
Jan 21, 2006·Science·Tarun M KapoorAlexey Khodjakov
Feb 6, 2007·Current Biology : CB·Amity L Manning, Duane A Compton
Feb 13, 2008·IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society·P ThévenazM Unser
Sep 24, 2008·The Journal of Cell Biology·Dileep VarmaRichard B Vallee
Dec 9, 2008·Nature Cell Biology·Samuel F BakhoumDuane A Compton
Jan 1, 2009·Molecular Biology of the Cell·Shang CaiClaire E Walczak
Jun 16, 2009·Nature Cell Biology·Shang CaiClaire E Walczak
Jun 24, 2009·Nature Reviews. Molecular Cell Biology·Andrew J Holland, Don W Cleveland
Jan 14, 2010·Nature Reviews. Molecular Cell Biology·Claire E WalczakAlexey Khodjakov
Feb 20, 2010·Proceedings of the National Academy of Sciences of the United States of America·Kenneth W WoodJeffrey R Jackson
Sep 5, 2012·The Journal of Cell Biology·Cornelia WandkeStephan Geley
Sep 22, 2012·Journal of Biochemistry·Kozo Tanaka
Apr 17, 2013·The Journal of Cell Biology·Jonne A RaaijmakersRené H Medema
Aug 21, 2013·Nature Cell Biology·Nikita GudimchukEkaterina L Grishchuk

❮ Previous
Next ❯

Citations

Jan 24, 2016·Biochimica Et Biophysica Acta·Hyuk-Joon JeonJeong Su Oh
Jan 21, 2016·Trends in Cell Biology·Hideki Yokoyama
Jun 28, 2016·Trends in Cell Biology·Marin Barisic, Helder Maiato
Oct 25, 2017·Molecular and Cellular Biology·Manpreet KaurSandeep Saxena
May 18, 2018·Journal of Molecular Cell Biology·Zhixiong DongWei Jiang
Feb 10, 2019·Biomolecules·Margarida Moura, Carlos Conde
Apr 30, 2020·Essays in Biochemistry·Benjamin Craske, Julie P I Welburn
Feb 22, 2017·Biology·Helder MaiatoMarin Barisic
Jul 18, 2017·Molecular Cell·Tadasu NozakiKazuhiro Maeshima
Sep 4, 2015·Journal of Cell Science·Philip Auckland, Andrew D McAinsh
May 15, 2021·The Journal of Cell Biology·Kenji IemuraKozo Tanaka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.