Chromophore-Assisted Light Inactivation of the V-ATPase V0c Subunit Inhibits Neurotransmitter Release Downstream of Synaptic Vesicle Acidification

Molecular Neurobiology
Sylvain RamaOussama El Far

Abstract

Synaptic vesicle proton V-ATPase is an essential component in synaptic vesicle function. Active acidification of synaptic vesicles, triggered by the V-ATPase, is necessary for neurotransmitter storage. Independently from its proton transport activity, an additional important function of the membrane-embedded sector of the V-ATPase has been uncovered over recent years. Subunits a and c of the membrane sector of this multi-molecular complex have been shown to interact with SNARE proteins and to be involved in modulating neurotransmitter release. The c-subunit interacts with the v-SNARE VAMP2 and facilitates neurotransmission. In this study, we used chromophore-assisted light inactivation and monitored the consequences on neurotransmission on line in CA3 pyramidal neurons. We show that V-ATPase c-subunit V0c is a key element in modulating neurotransmission and that its specific inactivation rapidly inhibited neurotransmission.

References

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Citations

Jan 26, 2020·IUBMB Life·Sindhuja Gowrisankaran, Ira Milosevic
Dec 20, 2020·Cellular and Molecular Life Sciences : CMLS·Enrico CastroflorioPeter L Oliver
Apr 13, 2021·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Kiwamu Takemoto

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Software Mentioned

LabView
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