PMID: 375085Mar 1, 1979Paper

Chromosomal aberration tests on 29 chemicals combined with S9 mix in vitro

Mutation Research
A MatsuokaM Ishidate

Abstract

A metabolic activation system with rat-liver microsome fraction plus cofactors (S9 mix) was applied to chromosomal aberration tests in vitro for the screening of chemical mutagens or carcinogens in the environment. Dialkylnitrosamines only induced chromosomal aberrations in Chinese hamster cells (CHL) when treated with S9 mix. The incidence of chromosomal aberrations in CHL varied with experimental conditions, e.g. incubation time, recovery time, components of S9 mic and inducers used for preparation of S9. For dimethylnitrosamine (DMN), the maximal incidence was obtained when the cells were incubated with S9 mix for 3 h and harvested 24 h after treatment. Therefore, this system (3 h incubation and 24 h recovery) was routinely applied to further screening of other chemicals with S9 prepared from PCB-pretreated rats. 10 carcinogens (e.g. 7,12-dimethylbenz[a]anthracene, benzo[a]pyrene, quinoline, etc.) out of 16 induced aberrations when they were treated with S9 mix, whereas the remaining 6 carcinogens (e.g., 3-methyl-cholanthrene, 4-o-tolylazo-o-toluidine, etc.) induced few or no aberrations even after activation. Two insecticides, allethrin and diazinon, were strongly positive at relatively low doses only when they were activat...Continue Reading

References

Mar 1, 1978·Mutation Research·E Zeiger, A T Sheldon
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J McCannB N Ames
Aug 15, 1975·International Journal of Cancer. Journal International Du Cancer·H F Stich, B A Laishes

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Citations

Mar 29, 2000·Environmental and Molecular Mutagenesis·R R TiceY F Sasaki
Nov 1, 1980·Archives of Toxicology·M B Roberfroid
Nov 1, 1985·Archives of Environmental Contamination and Toxicology·M R BleavinsJ E Wiley
Oct 1, 1986·Bulletin of Environmental Contamination and Toxicology·D LopezE Carrascal
Dec 1, 1994·Mutation Research·D Scott, R J Preston
Dec 1, 1989·Mutation Research·I ClietA Cordier
Jan 1, 1989·Mutation Research·V F GarryJ K Wiencke
Jan 1, 1989·Mutation Research·T MoritaK Okumura
Dec 17, 1990·Toxicology·M L WeinerL L Yang
Feb 22, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Y W ChengC F Wang

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