PMID: 270Aug 1, 1975

Chromosomal basis of the merozygosity in a partially deploid mutant of Pneumococcus

Genetics
M L Ledbetter, R D Hotchkiss

Abstract

A sulfonamide-resistant mutant of pneumococcus, sulr-c, displays a genetic instability, regularly segregating to wild type. DNA extracts of derivatives of the strain possess transforming activities for both the mutant and wild-type alleles, establishing that the strain is a partial diploid. The linkage of sulr-c to strr-61, a stable chromosomal marker, was established, thus defining a chromosomal locus for sulr-c. DNA isolated from sulr-c cells transforms two mutant recipient strains at the same low efficiency as it does a wild-type recipient, although the mutant property of these strains makes them capable of integrating classical "low-efficiency" donor markers equally as efficiently as "high efficiency" markers. Hence sulr-c must have a different basis for its low efficiency than do classical low efficiency point mutations. We suggest that the DNA in the region of the sulr-c mutation has a structural abnormality which leads both to its frequent segregation during growth and its difficulty in efficiently mediating genetic transformation.

Related Concepts

Genome Mapping
Chromosomes, Bacterial
Crosses, Genetic
Diploid Cell
DNA Replication
Antibiotic Resistance, Microbial
Haploid Cell
Genetic Linkage Analysis
Streptococcus pneumoniae
Sulfonamides

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