Chromosomal distribution of PcG proteins during Drosophila development.

PLoS Biology
Nicolas NègreGiacomo Cavalli

Abstract

Polycomb group (PcG) proteins are able to maintain the memory of silent transcriptional states of homeotic genes throughout development. In Drosophila, they form multimeric complexes that bind to specific DNA regulatory elements named PcG response elements (PREs). To date, few PREs have been identified and the chromosomal distribution of PcG proteins during development is unknown. We used chromatin immunoprecipitation (ChIP) with genomic tiling path microarrays to analyze the binding profile of the PcG proteins Polycomb (PC) and Polyhomeotic (PH) across 10 Mb of euchromatin. We also analyzed the distribution of GAGA factor (GAF), a sequence-specific DNA binding protein that is found at most previously identified PREs. Our data show that PC and PH often bind to clustered regions within large loci that encode transcription factors which play multiple roles in developmental patterning and in the regulation of cell proliferation. GAF co-localizes with PC and PH to a limited extent, suggesting that GAF is not a necessary component of chromatin at PREs. Finally, the chromosome-association profile of PC and PH changes during development, suggesting that the function of these proteins in the regulation of some of their target genes mig...Continue Reading

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Citations

Nov 29, 2013·Nucleic Acids Research·Arindam BasuMichael L Atchison
Aug 2, 2006·Proceedings of the National Academy of Sciences of the United States of America·Celine MoormanBas van Steensel
Oct 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Christian BeiselRenato Paro
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Apr 19, 2007·PLoS Biology·Xiaoyu ZhangSteven E Jacobsen
Aug 25, 2007·Genetics·Andrés Gaytán de Ayala AlonsoJürg Müller
Mar 29, 2008·PLoS Genetics·Elzo de WitBas van Steensel
Sep 6, 2008·PLoS Genetics·Camilla KwongRobert White
Dec 5, 2008·PLoS Biology·Vijay K TiwariStephen B Baylin
Dec 17, 2008·BMC Molecular Biology·Mo LiHaini N Cai
Jan 16, 2009·PLoS Biology·Bernd SchuettengruberGiacomo Cavalli
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Methods Mentioned

BETA
ubiquitination
transgenic
immunoprecipiation
chip
transfection
DamID
chips
PCR

Software Mentioned

GenePix Pro
Omnigrid
RDAM
Excel
Adobe Photoshop
PREdictor

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