Abstract
Squamous cell carcinoma of the head and neck and its subset, oral squamous cell carcinoma (OSCC), arise through a multistep process of genetic alterations as a result of exposure to environmental agents, such as tobacco smoke, alcoholic beverages, and viruses, including human papillomavirus. We and others have shown that the karyotypes of OSCC are near-triploid and contain multiple structural and numerical abnormalities. However, despite a background of clonal chromosomal aberrations, individual cells within a culture express many nonclonal numerical and structural abnormalities, termed chromosomal instability (CIN). To evaluate CIN in oral cancer cells, we isolated clones from two OSCC cell lines and carried out classical cytogenetic analysis, fluorescence in situ hybridization using centromere-specific probes, and spectral karyotyping. We observed variation in chromosome number within clones and between clones of the same cell line. Although similar numbers of centromeric signals for a particular chromosome were present, "homologs" of a chromosome varied structurally from cell to cell (marker chromosome evolution) as documented by classical and spectral karyotyping. In addition to the numerical chromosome variations within a ...Continue Reading
References
Jan 1, 1992·Genes, Chromosomes & Cancer·T RiedM Lipp
Feb 1, 1990·Genetics·F SpencerP Hieter
Jun 1, 1988·The Journal of Cell Biology·T C HuffakerD Botstein
Feb 1, 1995·The Histochemical Journal·K NylanderG Roos
Jan 1, 1993·Journal of Cellular Biochemistry. Supplement·T E CareyD L Van Dyke
Dec 15, 1995·Cytometry·S E ShackneyC A Smith
Apr 1, 1997·Nature Genetics·T VeldmanT Ried
Apr 18, 1997·Cell·A CoquelleM Debatisse
Apr 10, 1997·Nature·C LengauerB Vogelstein
Apr 1, 1998·Nature·D P CahillB Vogelstein
Jan 1, 1999·Nature·C LengauerB Vogelstein
Aug 17, 1999·Seminars in Cancer Biology·G A Pihan, S J Doxsey
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·W S SaundersS M Gollin
May 29, 2000·Genes, Chromosomes & Cancer·M I ShusterS M Gollin
Jan 9, 2001·Trends in Cell Biology·B R Brinkley
Jun 29, 2001·Head & Neck·S M Gollin
Oct 25, 2001·Proceedings of the National Academy of Sciences of the United States of America·D GisselssonN Mandahl
Jan 5, 2002·The New England Journal of Medicine·A ForastiereD Sidransky
Feb 19, 2002·Cancer Genetics and Cytogenetics·Charlotte JinFredrik Mertens
Feb 19, 2002·Oncogene·Jennifer A Hackett, Carol W Greider
May 3, 2002·Leukemia·A KrämerA D Ho
Jun 28, 2002·Cancer Cell·Asaf HellmanBatsheva Kerem
Jul 11, 2002·British Journal of Cancer·D GisselssonC Jin
Oct 24, 2002·Human Molecular Genetics·Marina CiulloMichelle Debatisse
Nov 5, 2002·Nature Reviews. Cancer·Erich A Nigg
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Lawrence A LoebJon P Anderson
Jul 23, 2003·Human Mutation·Cheryl A TelmerJonathan W Jarvik
Aug 14, 2003·Cell·Craig H BassingFrederick W Alt
Aug 14, 2003·Cell·Arkady CelesteAndré Nussenzweig
Aug 19, 2003·Nature Genetics·Donna G AlbertsonJoe W Gray
Dec 20, 2003·Current Opinion in Oncology·Susanne M Gollin
Mar 26, 2004·Cancer Genetics and Cytogenetics·Janet E ReingWilliam S Saunders
Aug 1, 1939·Proceedings of the National Academy of Sciences of the United States of America·B McClintock
Citations
Aug 10, 2006·Chromosoma·Shamini SelvarajahMaria Zielenska
May 14, 2010·Medical Oncology·Dongxia YeZhiyuan Zhang
Feb 3, 2007·Cytogenetic and Genome Research·S C ReshmiS M Gollin
Jan 11, 2014·The Journal of Craniofacial Surgery·Ana Luíza Laguardia CantaruttiEliete Neves da Silva Guerra
Dec 2, 2005·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Marga NadalVirginia Nunes
Jul 9, 2008·Acta Oto-laryngologica·Rudolf ReiterAnja Pickhard
Oct 26, 2007·Cancer Letters·Eswary ThirthagiriIan C Paterson
Aug 8, 2007·Oral Oncology·Christa Lese MartinSusanne M Gollin
Nov 23, 2006·Oral Oncology·Jason S WhiteSusanne M Gollin
Feb 22, 2007·Head & Neck·Charles J LinStephen Y Lai
Jul 29, 2010·The British Journal of Dermatology·G Martín-EzquerraR M Pujol
Oct 20, 2010·Histopathology·Lucio MontebugnoliMaria Pia Foschini
Dec 23, 2004·Seminars in Cancer Biology·Susanne M Gollin
Dec 9, 2008·Cancer Genetics and Cytogenetics·Lin LiPeter H Duesberg
Jun 2, 2006·Cancer Genetics and Cytogenetics·Kenichiro UchidaKohsuke Sasaki
Sep 4, 2014·Genes, Chromosomes & Cancer·Susanne M Gollin
May 27, 2011·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·L MontebugnoliMaria P Foschini
Jul 30, 2005·Virology·Aloysius J KlingelhutzShivanand R Patil
Sep 7, 2006·Cytogenetic and Genome Research·E SchrockK Stout-Weider
Apr 29, 2016·Genes, Chromosomes & Cancer·Hatem O KasebSusanne M Gollin
Sep 14, 2016·Experimental Cell Research·Hatem O KasebSusanne M Gollin
Apr 19, 2006·Oncogene·L-C HsuS M Gollin
Jan 26, 2005·Journal of Dental Research·S C Reshmi, S M Gollin
Jan 7, 2006·Cancer Research·John P WittschiebenRichard D Wood
Sep 5, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jennifer R Grandis
Sep 5, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rudolf ReiterAxel Walch
May 1, 2021·Cancers·Thomas ParzefallMarkus Brunner