Apr 22, 2020

Structural analysis of SARS-CoV-2 and predictions of the human interactome

BioRxiv : the Preprint Server for Biology
A. VandelliGian Gaetano Tartaglia


Specific elements of viral genomes regulate interactions within host cells. Here, we calculated the secondary structure content of >2500 coronaviruses and computed >100000 human protein interactions with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We found that the 3 and 5 prime ends are the most structured elements in the viral genome and the 5 prime end has the strongest propensity to associate with human proteins. The domain encompassing nucleotides 23000-24000 is highly conserved both at the sequence and structural level, while the region upstream varies significantly. These two sequences code for a domain of the viral protein Spike S that interacts with the human receptor angiotensin-converting enzyme 2 (ACE2) and has the potential to bind sialic acids. Our predictions indicate that the first 1000 nucleotides in the 5 prime end can interact with proteins involved in viral RNA processing such as Double-stranded RNA-specific Editase 1 ADARB1, A-kinase anchor protein 8-like AKAP8L and ATP-dependent RNA Helicase DDX1, in addition to other high-confidence candidate partners. These interactions, previously reported to be implicated in HIV, reveal important information on host-virus interactions. The list of tra...Continue Reading

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Mentioned in this Paper

Severe Acute Respiratory Syndrome
Chromosome Structures
AN 1
Trees (plant)
Inversion Mutation Abnormality
Chromosomes, Human
Chromosomal Inversion Process
Chromosome Inversion

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