Apr 22, 2020

Structural analysis of SARS-CoV-2 and predictions of the human interactome

BioRxiv : the Preprint Server for Biology
A. VandelliGian Gaetano Tartaglia

Abstract

Specific elements of viral genomes regulate interactions within host cells. Here, we calculated the secondary structure content of >2500 coronaviruses and computed >100000 human protein interactions with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We found that the 3 and 5 prime ends are the most structured elements in the viral genome and the 5 prime end has the strongest propensity to associate with human proteins. The domain encompassing nucleotides 23000-24000 is highly conserved both at the sequence and structural level, while the region upstream varies significantly. These two sequences code for a domain of the viral protein Spike S that interacts with the human receptor angiotensin-converting enzyme 2 (ACE2) and has the potential to bind sialic acids. Our predictions indicate that the first 1000 nucleotides in the 5 prime end can interact with proteins involved in viral RNA processing such as Double-stranded RNA-specific Editase 1 ADARB1, A-kinase anchor protein 8-like AKAP8L and ATP-dependent RNA Helicase DDX1, in addition to other high-confidence candidate partners. These interactions, previously reported to be implicated in HIV, reveal important information on host-virus interactions. The list of tra...Continue Reading

  • References
  • Citations

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations

Citations

  • This paper may not have been cited yet.

Mentioned in this Paper

Severe Acute Respiratory Syndrome
Chromosome Structures
AN 1
Genome
Trees (plant)
Inversion Mutation Abnormality
Chromosomes, Human
Chromosomal Inversion Process
Genomics
Chromosome Inversion

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.