Chromosomal mapping and expression of the human cyr61 gene in tumour cells from the nervous system

Molecular Pathology : MP
C MartinerieB Perbal

Abstract

To characterise the human cyr61 gene (cyr61H) and determine its chromosomal locality. To compare expression of cyr61H in human tumour cell lines with that of two other structurally related genes, novH (nephroblastoma overexpressed gene) and CTGF (connective tissue growth factor), that are likely to play a role in the control of cell proliferation and differentiation. To isolate the human cyr61 gene, placental genomic and HeLa cDNA libraries were screened with murine cyr61 cDNA. The nucleotide sequence of the complete cyr61H cDNA was established. Both Southern blotting of a panel of somatic cell hybrids and in situ hybridisation on chromosomes were performed to map the cyr61H gene. Expression of cyr61H, novH, CTGF, and novH was analysed by northern blotting in both human neuroblastomas and glioblastoma cell lines. Genomic and cDNA clones encompassing the cyr61H gene were isolated and characterised. Comparison of mouse and human cyr61 sequences indicated that their genomic organisation is highly conserved. Alignment of coding sequences highlighted the conservation of cyr61 regions that might be critical for its biological function. The data showed that the cyr61H gene is assigned to chromosome 1p22.3 and that different levels of ...Continue Reading

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Jul 13, 2000·Biochemical and Biophysical Research Communications·L R FitzgeraldP Tsui
Apr 22, 2009·Journal of Cell Communication and Signaling·Andrew Leask
Jan 8, 1999·Comptes rendus de l'Académie des sciences. Série III, Sciences de la vie·B Y SuB Perbal
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