Chromosomal structure is altered by mutations that suppress or enhance position effect variegation

Chromosoma
S HayashiT A Grigliatti

Abstract

We examined the genetic, morphological, and molecular effects of position effect variegation in Drosophila, and the effects of mutations that either suppress [Su(var)] or enhance [E(var)] this phenomenon. All eight Su(var) mutations examined strongly suppress the inactivation of variegating alleles of the genes white [In(l) wm4], brown [In(2R) bwVDe2] and Stubble [T(2; 3) SbV]. The E(var) mutation enhances variegation of these loci. The chromosomal region 3C-E (26 bands) which includes the white locus is usually packaged as heterochromatin in salivary glands of the variegating strain wm4. Addition of any of the Su(var) mutations restores a more euchromatic morphology to this region. In situ hybridization to polytene chromosomes and DNA blot analyses of gene copy number demonstrate that the DNA of the w+ gene is less accessible to its probe in the variegating wm4 strain than it is in the wild-type or variegation-suppressed strains. Blot analysis of larval salivary gland DNA indicates that the white gene copy number does not vary among the strains. Hence, the differences in binding of the w+ gene probe in the variegating and variegation-suppressed strains reflect differences in chromosomal packaging rather than alterations in gen...Continue Reading

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Citations

Sep 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G Reuter, P Spierer
Dec 1, 1990·Chromosoma·M L Pardue, W Hennig
Feb 1, 1993·Trends in Genetics : TIG·C D ShafferS C Elgin
Feb 14, 1998·Gene·E Zuckerkandl
Nov 8, 2005·Genetics·Neville CobbeMargarete M S Heck
Apr 1, 1994·Current Opinion in Genetics & Development·G H Karpen
Dec 1, 1993·Current Opinion in Cell Biology·R Paro
Sep 12, 2001·Genome Génome / Conseil National De Recherches Canada·P Morcillo, R J MacIntyre
Aug 17, 2020·Cells·Prim B SinghPetr P Laktionov
Jul 14, 2017·Scientific Reports·Nevine A ShalabyMichael Buszczak

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