Chromosome aberrations in peripheral blood lymphocytes of high-risk HPV-infected women with HGSIL

Environmental and Molecular Mutagenesis
Rosa E Alvarez-RoseroCarlos H Sierra-Torres

Abstract

Genomic instability is one of the main characteristics of malignant tumors, including HPV-induced cervical cancer. The aim of this study was to explore the use of assessing chromosome aberrations (CA) in peripheral blood lymphocytes as a biomarker for genomic instability in high-risk HPV-infected women with high-grade squamous intraepithelial lesions (HGSIL). A total of 120 women were recruited for this study, following cytology/colposcopy evaluation and HPV DNA detection. The study groups consisted of 30 HPV(+) women with histologically confirmed cervical intraepithelial neoplasia grade 2/3 and 30 HPV(+) women with carcinoma in situ (CIS). Two control groups, including 30 women HPV(-) and 30 women HPV(+), were recruited among women who were reported as cytology negative. Lymphocyte cell cultures were established for 52 hr, and 100 complete metaphase cells were evaluated per subject for CA analysis. The results show that women with CIS had significantly higher frequencies of both aneuploidy (0.67 +/- 0.20 vs. 0.14 +/- 0.08, P = 0.020) and tetraploidy (0.88 +/- 0.23 vs. 0.17 +/- 0.08, P = 0.013) in comparison with HPV(-) controls. These findings suggest the usefulness of peripheral blood lymphocytes to detect genomic instability...Continue Reading

References

May 1, 1997·Cancer Genetics and Cytogenetics·N B Atkin
Apr 23, 2002·JAMA : the Journal of the American Medical Association·Diane SolomonUNKNOWN Bethesda 2001 Workshop
Jan 29, 2003·Environmental and Molecular Mutagenesis·Carlos H Sierra-TorresStephen K Tyring
Jun 17, 2003·Journal of the National Cancer Institute. Monographs·Mark Schiffman, Susanne Krüger Kjaer
Jul 4, 2003·Critical Reviews in Eukaryotic Gene Expression·Stefan Duensing, Karl Münger
Dec 4, 2003·Cancer Letters·Andreas WidschwendterChristian Marth
Jun 16, 2004·Current Opinion in Genetics & Development·Viji M DraviamPeter K Sorger
Jul 29, 2004·Mutation Research·Monica S Sierra-TorresCarlos H Sierra-Torres
Sep 14, 2004·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·H J YangH Y S Ngan
Oct 14, 2004·Journal of Virology·Karl MüngerKyungwon Huh
Dec 23, 2004·Seminars in Cancer Biology·Oliver SieberIan Tomlinson
Jan 15, 2005·Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer·Silvia Franceschi
Apr 30, 2005·Cancer Genetics and Cytogenetics·Elva I Cortés-GutiérrezRicardo M Cerda-Flores
Aug 27, 2005·Carcinogenesis·Andrew J OlaharskiDavid A Eastmond
Nov 1, 2005·Yonsei Medical Journal·Young Tae Kim, Min Zhao
Jun 24, 2006·Environmental and Molecular Mutagenesis·Carlos H Sierra-TorresLeonora Orejuela-Aristizabal
Jan 26, 2007·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·Yu-Chi WeiTang-Yuan Chu

❮ Previous
Next ❯

Citations

Jul 16, 2014·T︠S︡itologii︠a︡ i genetika·E I Cortés-GutiérrezR M Cerda-Flores
Jul 2, 2011·Acta Obstetricia Et Gynecologica Scandinavica·Olivera Milošević-DjordjevićSlobodan Arsenijević

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.