PMID: 2507912Oct 1, 1989Paper

Chromosome analysis of small and large L5178Y mouse lymphoma cell colonies: comparison of trifluorothymidine-resistant and unselected cell colonies from mutagen-treated and control cultures

Mutation Research
W F BlazakW J Caspary

Abstract

Mutagenesis assays at the thymidine kinase (TK) locus in L5178Y mouse lymphoma cells frequently yield mutant colonies with a bimodal size distribution. The objectives of this study were to determine whether a relationship exists between mutant colony size and chromosomal aberrations and whether the colony-size distributions obtained from this assay can indicate the clastogenic activity of a test chemical. Cells from 8 different types of L5178Y mouse lymphoma cell colonies were examined for chromosomal abnormalities within 10 cell generations after colony isolation. The colonies included small (sigma) and large (lambda) unselected cell (UC) and trifluorothymidine-resistant (TFTr) colonies derived from TK +/- cell cultures treated with the solvent dimethyl sulfoxide (DMSO) or hycanthone methanesulfonate (HYC). Chromosome abnormalities were present in cells from 12% (7/60) of the UC colonies, but there was no apparent relationship between colony diameter and the presence of chromosomal abnormalities. Abnormalities affecting chromosome 11, which is believed to be the site of the TK gene, were not observed in cells from UC colonies. Abnormalities affecting chromosome 11 were observed only in cells from sigma-TFTr colonies irrespecti...Continue Reading

References

Jan 1, 1986·Environmental Mutagenesis·W F BlazakW J Caspary
Jan 1, 1988·Environmental and Molecular Mutagenesis·D B McGregorW J Caspary
Jan 1, 1987·Environmental Mutagenesis·M M MooreK L Dearfield
Jan 1, 1987·Environmental Mutagenesis·M M MooreD M DeMarini
Jun 1, 1983·Mutation Research·D CliveK H Mavournin

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Citations

Dec 13, 2000·Mutation Research·J Clements
Oct 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·D B McGregorW J Caspary
Feb 25, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jianyong WangMartha M Moore
Nov 30, 2004·Advances in Applied Microbiology·Kristien Mortelmans, Doppalapudi S Rupa
Mar 16, 2007·Environmental and Molecular Mutagenesis·Jianyong WangMartha M Moore
Jan 1, 1991·Environmental and Molecular Mutagenesis·D McGregorW J Caspary

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