PMID: 8947545Dec 1, 1996Paper

Chromosome architecture can dictate site-specific initiation of DNA replication in Xenopus egg extracts

The Journal of Cell Biology
S J LawlisD M Gilbert

Abstract

Xenopus egg extracts initiate DNA replication specifically at the dihydrofolate reductase (DHFR) origin locus with intact nuclei from late G1-phase CHO cells as a substrate, but at nonspecific sites when purified DNA is assembled by the extract into an embryonic nuclear structure. Here we show that late G1-phase CHO nuclei can be cycled through an in vitro Xenopus egg mitosis, resulting in the assembly of an embryonic nuclear envelope around G1-phase chromatin. Surprisingly, replication within these chimeric nuclei initiated at a novel specific site in the 5' region of the DHFR structural gene that does not function as an origin in cultured CHO cells. Preferential initiation at this unusual site required topoisomerase II-mediated chromosome condensation during mitosis. Nuclear envelope breakdown and reassembly in the absence of chromosome condensation resulted in nonspecific initiation. Introduction of condensed chromosomes from metaphase-arrested CHO cells directly into Xenopus egg extracts was sufficient to elicit assembly of chimeric nuclei and preferential initiation at this same site. These results demonstrate clearly that chromosome architecture can determine the sites of initiation of replication in Xenopus egg extracts,...Continue Reading

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Citations

Mar 10, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M L DePamphilis
Jun 27, 2000·Journal of Cellular Physiology·J A BoganM L Depamphilis
Nov 9, 2001·Biochemical and Biophysical Research Communications·T KubotaT Katayama
Dec 4, 2003·Journal of Molecular Biology·Anna ZawilakJolanta Zakrzewska-Czerwińska
Jul 4, 2002·Journal of Molecular Biology·John HerrickAaron Bensimon
Aug 28, 2003·Cell·Elizabeth S Egan, Matthew K Waldor
Jul 20, 2002·Trends in Biotechnology·Sandrine CaburetAaron Bensimon
Feb 18, 1998·Biochimie·S AutretS J Séror
Jun 26, 1998·Current Opinion in Cell Biology·P Pasero, S M Gasser
Jun 4, 1998·Current Opinion in Genetics & Development·D M Gilbert
Jun 15, 1999·Trends in Microbiology·S M Jazwinski
Feb 13, 2001·Nucleic Acids Research·S Kamath, M Leffak
Sep 16, 2003·Molecular and Cellular Biology·Kevin J Harvey, John Newport
Feb 18, 2003·Molecular and Cellular Biology·Guoqi LiuMichael Leffak
Dec 18, 2013·Chromosoma·Vladimir V SherstyukSuren M Zakian
Oct 18, 2015·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Joseph C SiefertChristopher L Sansam
Jul 11, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Daniela S Dimitrova
Jan 5, 2002·Differentiation; Research in Biological Diversity·R Flickinger
Mar 29, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Muktadir S HossainKazuhisa Sekimizu
Jan 20, 1999·Journal of Cellular Biochemistry. Supplement·M L DePamphilis
Aug 25, 1999·The Journal of Cell Biology·D S DimitrovaD M Gilbert
Jan 1, 1998·Journal of Cellular Biochemistry·Melvin L DePamphilis
Jan 27, 1998·Methods : a Companion to Methods in Enzymology·M L DePamphilis

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