Chromosome engineering of Escherichia coli for constitutive production of salvianic acid A

Microbial Cell Factories
Liang ZhouGuang-Rong Zhao

Abstract

Salvianic acid A (SAA), a valuable natural product from herbal plant Salvia miltiorrhiza, exhibits excellent antioxidant activities on food industries and efficacious therapeutic potential on cardiovascular diseases. Recently, production of SAA in engineered Escherichia coli was established via the artificial biosynthetic pathway of SAA on the multiple plasmids in our previous work. However, the plasmid-mediated system required to supplement expensive inducers and antibiotics during the fermentation process, restricting scale-up production of SAA. Microbial cell factory would be an attractive approach for constitutive production of SAA by chromosome engineering. The limited enzymatic reactions in SAA biosynthetic pathway from glucose were grouped into three modules, which were sequentially integrated into chromosome of engineered E. coli by λ Red homologous recombination method. With starting strain E. coli BAK5, in which the ptsG, pykF, pykA, pheA and tyrR genes were previously deleted, chassis strain BAK11 was constructed for constitutive production of precursor L-tyrosine by replacing the 17.7-kb mao-paa cluster with module 1 (P lacUV5 -aroG fbr -tyrA fbr -aroE) and the lacI gene with module 2 (P trc -glk-tktA-ppsA). The syn...Continue Reading

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Citations

Oct 21, 2019·Applied Microbiology and Biotechnology·Li-Qun JinYu-Guo Zheng
Nov 12, 2019·Biotechnology for Biofuels·Xue LiuGuang-Rong Zhao
Mar 11, 2020·Nature Ecology & Evolution·I TomanekC C Guet
May 11, 2018·Critical Reviews in Food Science and Nutrition·Min ShiGuoyin Kai
Jul 7, 2020·Journal of Agricultural and Food Chemistry·Yanan HouGuang-Rong Zhao
Jun 15, 2019·Journal of Agricultural and Food Chemistry·Jihua WuGuang-Rong Zhao
Nov 30, 2018·Journal of Agricultural and Food Chemistry·Ying ZhaoGuang-Rong Zhao

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