Chromosome Evolution in the Free-Living Flatworms: First Evidence of Intrachromosomal Rearrangements in Karyotype Evolution of Macrostomum lignano (Platyhelminthes, Macrostomida)

Genes
Kira S ZadesenetsNikolay B Rubtsov

Abstract

The free-living flatworm Macrostomum lignano is a hidden tetraploid. Its genome was formed by a recent whole genome duplication followed by chromosome fusions. Its karyotype (2n = 8) consists of a pair of large chromosomes (MLI1), which contain regions of all other chromosomes, and three pairs of small metacentric chromosomes. Comparison of MLI1 with metacentrics was performed by painting with microdissected DNA probes and fluorescent in situ hybridization of unique DNA fragments. Regions of MLI1 homologous to small metacentrics appeared to be contiguous. Besides the loss of DNA repeat clusters (pericentromeric and telomeric repeats and the 5S rDNA cluster) from MLI1, the difference between small metacentrics MLI2 and MLI4 and regions homologous to them in MLI1 were revealed. Abnormal karyotypes found in the inbred DV1/10 subline were analyzed, and structurally rearranged chromosomes were described with the painting technique, suggesting the mechanism of their origin. The revealed chromosomal rearrangements generate additional diversity, opening the way toward massive loss of duplicated genes from a duplicated genome. Our findings suggest that the karyotype of M. lignano is in the early stage of genome diploidization after whol...Continue Reading

References

Nov 10, 2000·Science·M Lynch, J S Conery
May 2, 2001·Nature Reviews. Genetics·K H Wolfe
Aug 6, 2003·Cytogenetic and Genome Research·N B RubtsovS M Zakian
Jun 10, 2004·Current Biology : CB·Ken Wolfe
Nov 24, 2005·Nature Reviews. Genetics·Luca Comai
Jan 16, 2007·Molecular Biology and Evolution·Marie Sémon, Kenneth H Wolfe
Nov 6, 2007·Cell·Sarah P Otto
Jan 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lukas SchärerDita B Vizoso
Jun 29, 2011·Parasitology International·Kira S ZadesenetsNikolay B Rubtsov
Jun 26, 2012·Nucleic Acids Research·Andreas UntergasserSteven G Rozen
Oct 25, 2013·Evolution; International Journal of Organic Evolution·Tim JanickeLukas Schärer
Mar 4, 2015·Nature·Anna M SelmeckiDavid Pellman
Sep 24, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kaja WasikMichael Schatz
Nov 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Jun InoueMutsumi Nishida
Apr 19, 2016·Nature Reviews. Genetics·Ricard Albalat, Cristian Cañestro
Sep 14, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Thomas LenormandChristoph R Haag
Apr 13, 2017·Genome Research·José M Martín-DuránAndreas Hejnol

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Citations

Nov 18, 2017·Genes·Thomas LiehrNikolai Rubtsov
Mar 12, 2020·EvoDevo·Jakub WudarskiEugene Berezikov
Mar 1, 2018·Aging Cell·Stijn MoutonEugene Berezikov
Jan 24, 2020·International Journal of Molecular Sciences·Kira S ZadesenetsNikolay B Rubtsov
Mar 5, 2021·Vavilovskiĭ Zhurnal Genetiki I Selekt︠s︡ii·K S Zadesenets, N B Rubtsov
Mar 5, 2021·Vavilovskiĭ Zhurnal Genetiki I Selekt︠s︡ii·K S Zadesenets, N B Rubtsov

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Methods Mentioned

BETA
dissection
PCR

Software Mentioned

Mli1dist
METASystems
Primer3
ISIS

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