Chromosome organization and replisome dynamics in Mycobacterium smegmatis

MBio
Isabella Santi, John D McKinney

Abstract

Subcellular organization of the bacterial nucleoid and spatiotemporal dynamics of DNA replication and segregation have been studied intensively, but the functional link between these processes remains poorly understood. Here we use quantitative time-lapse fluorescence microscopy for single-cell analysis of chromosome organization and DNA replisome dynamics in Mycobacterium smegmatis. We report that DNA replication takes place near midcell, where, following assembly of the replisome on the replication origin, the left and right replication forks colocalize throughout the replication cycle. From its initial position near the cell pole, a fluorescently tagged chromosomal locus (attB, 245° from the origin) moves rapidly to the replisome complex just before it is replicated. The newly duplicated attB loci then segregate to mirror-symmetric positions relative to midcell. Genetic ablation of ParB, a component of the ParABS chromosome segregation system, causes marked defects in chromosome organization, condensation, and segregation. ParB deficiency also results in mislocalization of the DNA replication machinery and SMC (structural maintenance of chromosome) protein. These observations suggest that ParB and SMC play important and over...Continue Reading

References

Nov 9, 2000·Molecular Microbiology·T WeitaoK Nordström
Feb 13, 2001·Molecular Cell·K P Lemon, A D Grossman
Sep 5, 2001·The EMBO Journal·R B JensenLucy Shapiro
Jun 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·Patrick H ViollierLucy Shapiro
Sep 1, 2005·Molecular Genetics and Genomics : MGG·Shun AdachiS Hiraga
Jul 5, 2006·Genes & Development·Xindan WangDavid J Sherratt
Sep 1, 2006·Molecular Microbiology·Melanie B Berkmen, A D Grossman
Mar 23, 2007·Molecular Microbiology·Heather Hendrickson, Jeffrey G Lawrence
Oct 2, 2007·Journal of Bacteriology·Jonathan LivnyMatthew K Waldor
Nov 6, 2007·Journal of Bacteriology·Carolin GüthleinBurkhard Springer
Apr 9, 2008·Cell·Rodrigo Reyes-LamotheDavid J Sherratt
Aug 5, 2008·Molecular Microbiology·David Bates
Jun 13, 2009·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·John F Marko
Feb 26, 2010·Cold Spring Harbor Perspectives in Biology·Esteban Toro, Lucy Shapiro
Jul 27, 2010·Nature Cell Biology·Jerod L PtacinLucy Shapiro
Jun 10, 2011·Molecular Microbiology·Anita MinnenJan-Willem Veening
Jul 15, 2011·Molecular Microbiology·Zoya M PetrushenkoValentin V Rybenkov
Aug 23, 2011·Current Opinion in Chemical Biology·Jon M Kaguni
Sep 20, 2011·Molecular Microbiology·Monica A Schwartz, Lucy Shapiro
Dec 29, 2011·Frontiers in Bioscience (Landmark Edition)·Christophe PossozOlivier Espeli
Aug 3, 2012·Cold Spring Harbor Perspectives in Biology·Dean JacksonDavid Z Rudner
Jan 5, 2013·Science·Yuichi WakamotoJohn D McKinney
Feb 13, 2013·Nature Reviews. Genetics·Xindan WangDavid Z Rudner
May 10, 2013·PLoS Genetics·Isabelle Vallet-Gely, Frédéric Boccard
Sep 17, 2013·Nature Communications·Isabella SantiJohn D McKinney
Oct 15, 2013·FEMS Microbiology Reviews·Sophie Nolivos, David J Sherratt
Jan 21, 2014·Current Biology : CB·Xindan WangDavid Z Rudner
Feb 20, 2014·Frontiers in Microbiology·Leigh G MonahanElizabeth J Harry
May 27, 2014·ELife·Hoong Chuin LimChristine Jacobs-Wagner

Citations

Nov 10, 2015·Journal of Drug Targeting·Huan WangJianping Xie
Jun 23, 2015·Frontiers in Microbiology·Thomas R Beattie, Rodrigo Reyes-Lamothe
Apr 14, 2017·Molecular Biology and Evolution·Jelena Repar, Tobias Warnecke
Mar 7, 2017·Scientific Reports·Damian TrojanowskiJolanta Zakrzewska-Czerwińska
Jun 27, 2017·Nature Microbiology·Haig A EskandarianGeorg E Fantner
May 19, 2017·Molecular Microbiology·Katarzyna GindaJohn D McKinney
Nov 9, 2017·MBio·Joanna HołówkaJolanta Zakrzewska-Czerwińska
Nov 28, 2017·PLoS Genetics·Katherine M MannChristina L Stallings
Jun 10, 2019·Chembiochem : a European Journal of Chemical Biology·Rebecca L TarnopolAnthony G Vecchiarelli
Jun 17, 2020·Open Biology·Adam S B Jalal, Tung B K Le
Jul 28, 2017·Frontiers in Cell and Developmental Biology·Miles PriestmanVahid Shahrezaei
May 3, 2018·Pathogens and Disease·Nathan J MacGilvary, Shumin Tan
Dec 12, 2018·Frontiers in Microbiology·Damian TrojanowskiJolanta Zakrzewska-Czerwińska
Jul 25, 2019·Nature Communications·Alexandra JungMartin Thanbichler
May 14, 2016·G3 : Genes - Genomes - Genetics·Supriya Khedkar, Aswin Sai Narain Seshasayee
Nov 25, 2020·Journal of Bacteriology·Jemila C KesterSarah M Fortune
Feb 5, 2021·Scientific Reports·Marta KołodziejJolanta Zakrzewska-Czerwińska
Sep 12, 2020·Current Biology : CB·Aditya C BandekarChristopher M Sassetti
Nov 14, 2016·Microbiology Spectrum·Neeraj DharGiulia Manina

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.