Chromosome Painting Based on Bulked Oligonucleotides in Cotton

Frontiers in Plant Science
Yuling LiuRenhai Peng

Abstract

Chromosome painting is one of the key technologies in cytogenetic research, which can accurately identify chromosomes or chromosome regions. Oligonucleotide (oligo) probes designed based on genome sequences have both flexibility and specificity, which would be ideal probes for fluorescence in situ hybridization (FISH) analysis of genome structure. In this study, the bulked oligos of the two arms of chromosome seven of cotton were developed based on the genome sequence of Gossypium raimondii (DD, 2n = 2× = 26), and each arm contains 12,544 oligos. Chromosome seven was easily identified in both D genome and AD genome cotton species using the bulked chromosome-specific painting probes. Together with 45S ribosomal DNA (rDNA) probe, the chromosome-specific painting probe was also successfully used to correct the chromosomal localization of 45S rDNA in G. raimondii. The study reveals that bulked oligos specific to a chromosome is a useful tool for chromosome painting in cotton.

References

Jan 1, 1978·Annual Review of Genetics·D E Comings
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D PinkelJ Gray
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·J F WendelT Seelanan
Jan 1, 1996·Chromosoma·J FuchsI Schubert
May 29, 2003·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Martin A LysakIngo Schubert
Dec 17, 2005·Annals of Botany·Robert HasterokJolanta Maluszynska
Apr 13, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Kai WangTianzhen Zhang
Jul 11, 2006·The Plant Journal : for Cell and Molecular Biology·Piotr A ZiolkowskiJan Sadowski
Nov 18, 2006·Genome Génome / Conseil National De Recherches Canada·Jiming Jiang, Bikram S Gill
Apr 5, 2007·Nature Protocols·Willem RensMalcolm Ferguson-Smith
Jun 14, 1929·Science·B McClintock
Nov 17, 2007·Nature Reviews. Genetics·Malcolm A Ferguson-Smith, Vladimir Trifonov
Oct 7, 2008·The Plant Cell·Terezie Mandáková, Martin A Lysak
May 27, 2010·Cytogenetic and Genome Research·M A LysakE Lacombe
Apr 6, 2013·Methods in Molecular Biology·Martin A Lysak, Terezie Mandáková
Jun 14, 2013·Journal of Integrative Plant Biology·Qiong WuKunbo Wang
Dec 21, 2017·Cytogenetic and Genome Research·Manman QuYonghua Han
Jun 9, 2018·BMC Plant Biology·Lili HouHengxiu Yu
Nov 30, 2018·BMC Plant Biology·Hejun LuKunbo Wang
Jan 19, 2019·Proceedings of the National Academy of Sciences of the United States of America·Patrice S AlbertJames A Birchler
Mar 11, 2019·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Jiming Jiang
Sep 9, 2019·The Plant Journal : for Cell and Molecular Biology·Xiaoyu LiuZhiyun Gong
Nov 28, 2019·The New Phytologist·Robert HasterokGlyn Jenkins

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Datasets Mentioned

BETA
U12718

Methods Mentioned

BETA
in vitro transcription

Software Mentioned

ChorusNGSselect
Chorus2
ITS
ChorusNGSfilter
MetaSystems Isis

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.