Chromosome Segregation Fidelity in Epithelia Requires Tissue Architecture

Cell
Kristin A KnouseAngelika Amon

Abstract

Much of our understanding of chromosome segregation is based on cell culture systems. Here, we examine the importance of the tissue environment for chromosome segregation by comparing chromosome segregation fidelity across several primary cell types in native and nonnative contexts. We discover that epithelial cells have increased chromosome missegregation outside of their native tissues. Using organoid culture systems, we show that tissue architecture, specifically integrin function, is required for accurate chromosome segregation. We find that tissue architecture enhances the correction of merotelic microtubule-kinetochore attachments, and this is especially important for maintaining chromosome stability in the polyploid liver. We propose that disruption of tissue architecture could underlie the widespread chromosome instability across epithelial cancers. Moreover, our findings highlight the extent to which extracellular context can influence intrinsic cellular processes and the limitations of cell culture systems for studying cells that naturally function within a tissue.

Citations

Mar 15, 2019·Molecular Biology of the Cell·Iva M Tolić, Daniel W Gerlich
May 11, 2019·Cell Cycle·Masatoshi Hara, Tatsuo Fukagawa
May 18, 2019·Cell Cycle·Shuyuan ZhangHao Zhu
Jun 8, 2019·Journal of Molecular Cell Biology·Xu LiuXuebiao Yao
May 1, 2019·Nature Genetics·Ana C F BolhaqueiroGeert J P L Kops
Apr 3, 2020·Journal of Molecular Cell Biology·Lulu Sun, Lijian Hui
Aug 2, 2019·World Journal of Gastrointestinal Oncology·Miranda LinJoseph Kim
Sep 22, 2019·International Journal of Molecular Sciences·David L Newman, Stephen L Gregory
Apr 4, 2020·Nature Reviews. Gastroenterology & Hepatology·Romain DonneChantal Desdouets
Jun 12, 2019·Nature·Sarah C Johnson, Sarah E McClelland
Feb 15, 2020·Nature Communications·Louisa NelsonStephen S Taylor
Nov 22, 2018·Disease Models & Mechanisms·Robert E HyndsSam M Janes
Jan 4, 2019·Nature Cell Biology·Narendra Kumar Chunduri, Zuzana Storchová
Dec 24, 2018·Nature Reviews. Cancer·Uri Ben-DavidTodd R Golub
Nov 19, 2019·The Journal of Clinical Investigation·Cynthia J SiebenJan M van Deursen
Aug 29, 2019·Annual Review of Cell and Developmental Biology·Melissa A PickettJessica L Feldman
Apr 8, 2020·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rüdiger BraunThomas Ried
Jan 15, 2021·Current Opinion in Genetics & Development·Eduardo A Maury, Christopher A Walsh
Oct 27, 2020·Frontiers in Cell and Developmental Biology·Selwin K WuMichael Smutny
Feb 16, 2021·Frontiers in Bioengineering and Biotechnology·Brandon D RiehlJung Yul Lim
Mar 21, 2021·Communications Biology·Maia Al-MasriLuke McCaffrey
Mar 12, 2020·Cell Reports·Julia RohrbergAndrei Goga
Mar 27, 2021·Nature Communications·Miryam MüllerThomas G Bird
Mar 25, 2021·Cell Reports·Akshay NarkarRong Li
Mar 26, 2021·Seminars in Liver Disease·Patrick D Wilkinson, Andrew W Duncan
Apr 1, 2019·The American Journal of Pathology·Patrick D WilkinsonAndrew W Duncan
Apr 7, 2021·Reproductive Biomedicine Online·Thomas T F HuangAaron Ohta
Apr 28, 2021·The Journal of Cell Biology·Geng-Yuan ChenMichael A Lampson
Jun 24, 2021·Cell Reports·Ximena ContrerasSimon Hippenmeyer
Jul 7, 2021·Journal of Hepatology·Valentina C SladkyAndreas Villunger
Jul 24, 2021·Scientific Reports·Rachel NewcombJames V Alvarez
Jul 14, 2021·Nature Communications·M L RichterC P Martinez-Jimenez
Sep 22, 2021·Journal of Molecular Biology·Shalaka PatilKundan Sengupta
Oct 5, 2021·Endocrinology·Katrina M PiemonteRuth A Keri

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.