Feb 15, 2011

Chromosome-specific segmentation revealed by structural analysis of individually isolated chromosomes

Genes, Chromosomes & Cancer
Kunio KitadaS Aikawa

Abstract

Analysis of structural rearrangements at the individual chromosomal level is still technologically challenging. Here we optimized a chromosome isolation method using fluorescent marker-assisted laser-capture and laser-beam microdissection and applied it to structural analysis of two aberrant chromosomes found in a lung cancer cell line. A high-density array-comparative genomic hybridization (array-CGH) analysis of DNA samples prepared from each of the chromosomes revealed that these two chromosomes contained 296 and 263 segments, respectively, ranging from 1.5 kb to 784.3 kb in size, derived from different portions of chromosome 8. Among these segments, 242 were common in both aberrant chromosomes, but 75 were found to be chromosome-specific. Sequences of 263 junction sites connecting the ends of segments were determined using a PCR/Sanger-sequencing procedure. Overlapping microhomologies were found at 169 junction sites. Junction partners came from various portions of chromosome 8 and no biased pattern in the positional distribution of junction partners was detected. These structural characteristics suggested the occurrence of random fragmentation of the entire chromosome 8 followed by random rejoining of these fragments. Base...Continue Reading

Mentioned in this Paper

Flow Cytometry
Fluorescent in Situ Hybridization
Biologic Segmentation
Abnormal Fragmented Structure
Array-Based Comparative Genomic Hybridization
DNA, Neoplasm
Fluorescent stain
Autosome Abnormalities
Sequencing
Massively-Parallel Sequencing

Related Feeds

Cancer Sequencing

Several sequencing approaches are employed to understand and examine tumor development and progression. These include whole genome as well as RNA sequencing. Here is the latest research on cancer sequencing.