Chromosome transfer from F-lac+ strains of Escherichia coli K-12 mutant at recA, recB, or recC.

Journal of Bacteriology
B M Wilkins

Abstract

The frequency of chromosome transfer from various recombination-deficient F-lac(+) donor strains was estimated by standardizing the yield of conjugants receiving a male chromosomal marker against the level of episome transfer in the mating mixture. The efficiency of chromosome transfer from newly formed F-lac(+) cells carrying recB21 or recC22 was more than 50% of the wild-type value, although it was about 10 and 20%, respectively, if the male cell lines had become established. In contrast, recA13 donors transmitted the chromosome with less than 10(-4) of the normal frequency. If chromosome transfer from F-lac(+) strains reflects the cutting and subsequent joining of homologous single strands of episomal and chromosomal deoxyribonucleic acid by recombination, these results imply that the completed unions are not made in recA cells, but can be effected with more than 50% of normal efficiency in newly formed partial diploids mutant at either recB or recC. Thus, the defective stage in recA mutants may precede strand joining, whereas the deficiency in recB or recC cells may involve a later step in recombinant formation.

References

Jan 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·M Gellert
Dec 1, 1967·Bacteriological Reviews·A L Taylor, C D Trotter
Oct 1, 1967·Journal of Cellular Physiology·A J Clark
May 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·B Low
Jan 1, 1968·Cold Spring Harbor Symposia on Quantitative Biology·W D Rupp, G Ihler
Jan 1, 1968·Cold Spring Harbor Symposia on Quantitative Biology·M Ohki, J Tomizawa
Apr 1, 1966·Journal of Molecular Biology·J D Gross, L G Caro
Jul 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·M L GefterJ Hurwitz
Mar 1, 1960·Journal of Bacteriology·E A ADELBERG, S N BURNS
Feb 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·A J CLARK, A D MARGULIES
Jun 1, 1965·Bacteriological Reviews·E A ADELBERG, J PITTARD

❮ Previous
Next ❯

Citations

Jan 16, 1976·Molecular & General Genetics : MGG·K A Stacey, R G Lloyd
Feb 1, 1991·Molecular & General Genetics : MGG·F BensonR G Lloyd
Dec 5, 2008·Microbiology and Molecular Biology Reviews : MMBR·Mark S Dillingham, Stephen C Kowalczykowski
Jan 1, 1973·Zeitschrift für allgemeine Mikrobiologie·H Tschäpe
Oct 13, 1969·Molecular & General Genetics : MGG·S R Palchoudhury, V N Iyer
May 1, 1970·Biological Reviews of the Cambridge Philosophical Society·H L Whitehouse
Mar 19, 2019·Molecular Microbiology·Nicklas A HamiltonJustin Courcelle
Jan 1, 1973·Molecular & General Genetics : MGG·R M BenbowP Howard-Flanders
May 1, 1972·Journal of Bacteriology·J D Hall, P Howard-Flanders
Dec 1, 1972·Bacteriological Reviews·K B Low
Jan 1, 1975·Journal of Bacteriology·N S Willetts
Nov 1, 1972·Journal of Bacteriology·N S Willetts
Jan 28, 1972·Journal of Molecular Biology·H Yamagata, H Uchida
Jan 1, 1979·Plasmid·B W Holloway
Feb 25, 1983·Journal of Molecular Biology·J W ChaseJ J Sninsky

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.