Chromosome walking in the Petunia inflata self-incompatibility (S-) locus and gene identification in an 881-kb contig containing S2-RNase

Plant Molecular Biology
Yan WangTeh-Hui Kao

Abstract

Self-incompatibility (SI) in the Solanaceae, Rosaceae and Scrophulariaceae is controlled by the polymorphic S locus, which contains two separate genes encoding pollen and pistil determinants in SI interactions. The S-RNase gene encodes the pistil determinant, whereas the pollen determinant gene, named the pollen S gene, has not yet been identified. Here, we set out to construct an integrated genetic and physical map of the S locus of Petunia inflata and identify any additional genes located at this locus. We first conducted chromosome walking at the S2 locus using BAC clones that contained either S2-RNase or one of the nine markers tightly linked to the S locus. Ten separate contigs were constructed, which collectively spanned 4.4 Mb. To identify additional genes located at the S2 locus, a 328-kb region (part of an 881-kb BAC contig) containing S2-RNase was completely sequenced. Approximately 76% of the region contained repetitive sequences, including transposon-like sequences. Other than S2-RNase, an F-box gene, named PiSLF2 (S2-allele of P. inflata S-locus F-box gene), was the only predicted gene whose deduced amino acid sequence was similar to the sequences of known proteins in the database. Two different cDNA selection meth...Continue Reading

Citations

Mar 14, 2009·Molecular Genetics and Genomics : MGG·Jonathan J D LabonneJoel S Shore
Jan 5, 2008·Plant Molecular Biology·Kazuma OkadaTakeshi Takasaki-Yasuda
May 9, 2012·Journal of Experimental Botany·Paolo De FranceschiJavier Sanzol
Feb 22, 2005·The New Phytologist·Marcy K Uyenoyama
Jul 25, 2008·Genome Génome / Conseil National De Recherches Canada·Inge Van DaeleIsabel Roldán-Ruiz
Jul 7, 2009·Annual Review of Plant Biology·Yijing ZhangYongbiao Xue
Feb 24, 2015·Frontiers in Plant Science·Justin S WilliamsTeh-Hui Kao
Sep 1, 2016·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Javier Do CantoThomas Lubberstedt
Jul 19, 2005·Trends in Genetics : TIG·Ed Newbigin, Marcy K Uyenoyama
Aug 2, 2011·Annals of Botany·Bruce McClureCarlos Romero
Jan 5, 2011·Annals of Botany·Xiaoying MengTeh-hui Kao
Oct 10, 2006·Current Opinion in Plant Biology·Bruce McClure
Apr 12, 2005·Plant Molecular Biology·Lei WangYongbiao Xue
Sep 13, 2008·The Plant Journal : for Cell and Molecular Biology·Chun-Lei WangShao-Ling Zhang
Mar 1, 2012·Wiley Interdisciplinary Reviews. Developmental Biology·Ning Wang, Teh-Hui Kao
Mar 20, 2010·Biochemical Society Transactions·Thomas L SimsPratima Shrestha
Sep 21, 2013·Journal of Proteome Research·Subramanian SankaranarayananMarcus A Samuel
Mar 5, 2008·Current Opinion in Plant Biology·Mikkel Heide Schierup, Xavier Vekemans

❮ Previous
Next ❯

Related Concepts

Related Feeds

Artificial Chromosomes

Artificial chromosomes are genetically engineered chromosomes derived from the DNA of a species. Discover the latest research on artificial chromosomes here.